文献类型： 外文期刊

第一作者： SHU Ben-shui

作者： SHU Ben-shui;YU Hai-kuo;DAI Jing-hua;XIE Zi-ge;LIN Jin-tian;QIAN Wan-qiang

作者机构：

关键词： Spodoptera frugiperda; reference genes; qPCR; stability evaluation; different experimental conditions

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2021 年 20 卷 9 期

页码：

收录情况： SCI

摘要： Real-time quantitative PCR (qPCR) is a reliable and widely used technique for analyzing the expression profiles of target genes in different species, and reference genes with stable expressions have been introduced for the normalization of the data. Therefore, stability evaluation should be considered as the initial step for qPCR experiments. The fall armyworm Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) is a polyphagous pest that consumes many plant species and seriously threatens corn production around the world. However, no studies thus far have examined the stability of reference genes in this pest. In this study, the expression profiles of the eight candidate reference genes of Actin, elongation factor 1 alpha (EF1 alpha), elongation factor 2 (EF2), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), ribosomal protein L3 (RPL3), ribosomal protein L13 (RPL13), alpha-tubulin (alpha -TUB), and beta-1-tubulin (beta -1-TUB) were obtained from S. frugiperda in different samples and the stability was evaluated by Delta Ct, BestKeeper, geNorm, NormFinder, and RefFinder methods. The results of pairwise variation (V) calculated by GeNorm indicated two reference genes could be selected for normalization. Therefore, the combinations of the most stable reference genes for different experimental conditions of S. frugiperda were shown as follows: EF2 and RPL13 for developmental stages, RPL3 and beta -1-TUB for larval tissue samples, EF2 and EF1 alpha for the larval samples treated with different temperatures, RPL3 and EF1 alpha for the larval samples under starvation stress, and RPL13 and EF1 alpha for all the samples. Our results lay the foundation for the normalization of qPCR analyses in S. frugiperda and could help guarantee the accuracy of subsequent research.

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