Overexpression of an aquaporin protein from Aspergillus glaucus confers salt tolerance in transgenic soybean

文献类型： 外文期刊

第一作者： Li, Feiwu

作者： Li, Feiwu;Zhang, Shi-Hong;Li, Feiwu;Yan, Wei;Xie, Yanbo;Tan, Xichang;Zhang, Ling;Ni, Hejia;Liu, Xiaodan

作者机构：

关键词： AgGlpF gene; Transgenic soybean; Salt tolerance

期刊名称：TRANSGENIC RESEARCH （ 影响因子：2.788； 五年影响因子：2.377 ）

ISSN： 0962-8819

年卷期： 2021 年 30 卷 6 期

页码：

收录情况： SCI

摘要： Salt stress is an important abiotic factor that causes severe losses in soybean yield and quality. Therefore, breeding salt-tolerant soybean germplasm resources via genetic engineering has gained importance. Aspergillus glaucus, a halophilic fungus that exhibits significant tolerance to salt, carries the gene AgGlpF. In this study, we used the soybean cotyledonary node transformation method to transfer the AgGlpF gene into the genome of the soybean variety Williams 82 to generate salt-tolerant transgenic soybean varieties. The results of PCR, Southern blot, ddPCR, and RT-PCR indicated that AgGlpF was successfully integrated into the soybean genome and stably expressed. When subjected to salt stress conditions via treatment with 250 mM NaCl for 3 d, the transgenic soybean plants showed significant tolerance compared with wild-type plants, which exhibited withering symptoms and leaf abscission after 9 d. The results of this study indicated that the transfer of AgGlpF into the genome of soybean plants produced transgenic soybean with significantly improved salt stress tolerance.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序