Microbial Community and Metabolome Analysis of the Porcine Intestinal Damage Model Induced by the IPEC-J2 Cell Culture-Adapted Porcine Deltacoronavirus (PDCoV) Infection

文献类型： 外文期刊

第一作者： Shi, Ying

作者： Shi, Ying;Li, Benqiang;Cheng, Jinghua;Tao, Jie;Tang, Pan;Jiao, Jiajie;Liu, Huili;Shi, Ying;Li, Benqiang;Cheng, Jinghua;Tao, Jie;Tang, Pan;Jiao, Jiajie;Liu, Huili;Shi, Ying;Li, Benqiang;Cheng, Jinghua;Tao, Jie;Tang, Pan;Jiao, Jiajie;Liu, Huili

作者机构：

关键词： porcine deltacoronavirus; intestinal damage; piglets; microbial community profiling; metabolome analysis

期刊名称：MICROORGANISMS （ 影响因子：4.5； 五年影响因子：4.8 ）

ISSN：

年卷期： 2024 年 12 卷 5 期

页码：

收录情况： SCI

摘要： This study was conducted to elucidate the intestinal damage induced by the IPEC-J2 cell culture-passaged PDCoV. The results showed that PDCoV disrupted the intestinal structure and increased intestinal permeability, causing abnormalities in mucosal pathology. Additionally, PDCoV induced an imbalance in the intestinal flora and disturbed its stability. Microbial community profiling revealed bacterial enrichment (e.g., Proteobacteria) and reduction (e.g., Firmicutes and Bacteroidetes) in the PDCoV-inoculated piglet model. In addition, metabolomics analysis indicated that 82 named differential metabolites were successfully quantified, including 37 up-regulated and 45 down-regulated metabolites. Chenodeoxycholic acid, sphingosine, and oleanolic aldehyde levels were reduced in PDCoV-inoculated piglets, while phenylacetylglycine and geranylgeranyl-PP levels were elevated. Correlation analysis indicated a negative correlation between Escherichia-Shigella and choline, succinic acid, creatine, phenyllactate, and hippuric acid. Meanwhile, Escherichia-Shigella was positively correlated with acetylcholine, L-Glutamicacid, and N-Acetylmuramate. Roseburia, Lachnospiraceae_UCG-010, Blautia, and Limosilactobacillus were negatively and positively correlated with sphingosine, respectively. These data suggested PDCoV-inoculated piglets exhibited significant taxonomic perturbations in the gut microbiome, which may result in a significantly altered metabolomic profile.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序