Construction of biocatalysts based on P450BM3 for the degradation of non-steroidal anti-inflammatory drugs

文献类型： 外文期刊

第一作者： Yang, Yadan

作者： Yang, Yadan;Zhang, Weikang;Wang, Fang;Li, Dong;Xu, Jiakun;Yang, Yadan;Zhang, Weikang;Meng, Xiangmin;Sun, Xiaochun;Wang, Fang;Xu, Jiakun

作者机构：

关键词： NSAID; P450BM3; Rational design; Biocatalysis; Degradation mechanism

期刊名称：JOURNAL OF HAZARDOUS MATERIALS （ 影响因子：11.3； 五年影响因子：12.4 ）

ISSN： 0304-3894

年卷期： 2024 年 480 卷

页码：

收录情况： SCI

摘要： Non-steroidal anti-inflammatory drugs (NSAIDs) are widespread pollutants in aquatic environments, posing significant risks to both ecosystems and human health due to their persistence and bioaccumulation. Effective and sustainable degradation methods are urgently required to address this environmental challenge. This study aims to design and optimize a cytochrome P450BM3-based biocatalyst for the rapid and efficient degradation of NSAIDs by direct chemical intervention and protein engineering. The novel biocatalyst achieved efficient biodegradation of four common NSAIDs. Notably, the F87I/T268D mutant achieved 99.22 % degradation of diclofenac (DCF) within 10 min, and degraded meloxicam (MEL) and phenylbutazone (PBZ) at rates of 98.86 % and 90.51 % within 5 min, respectively. Furthermore, the F87G mutant accomplished 99.08 % degradation of acetaminophen (APAP) within just 2 min. The catalytic properties of P450BM3 and its mutants were evaluated

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