Detection of gyromitrin-induced mushroom poisoning by an isotope-coded derivatization strategy combined with UPLC-MS/MS and its application

文献类型： 外文期刊

第一作者： Fan, Tingting

作者： Fan, Tingting;He, Xiangwei;Hengchao, E.;Zhang, Yanmei;Li, Xiaobei;Li, Xujiao;Yang, Xianli;Zhou, Changyan;Zhao, Zhiyong;Zhao, Xiaoyan;Zhao, Zhiyong

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关键词： Biomarker; Detection; Gyromitrin; Isotope-coded Derivatization; UPLC-MS/MS

期刊名称：MICROCHEMICAL JOURNAL （ 影响因子：4.8； 五年影响因子：4.5 ）

ISSN： 0026-265X

年卷期： 2024 年 199 卷

页码：

收录情况： SCI

摘要： Gyromitrin is the main toxic metabolite produced from the false morel such as Gyromitra esculenta, which has a long history of causing food poisoning. However, how to identify the gyromitrin-induced poisoning is difficult because the biomarker of gyromitrin in vivo is still unclear. In this study, a novel isotope-coded method combined with the derivatization strategy was first developed for the accurate quantification of gyromitrin in urine and plasma by ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). The major parameters affecting the derivatization efficiency were systematically investigated, and the p-tolualdehyde-d4 was employed to form the isotope internal standard. The developed method was fully validated with the limit of detection (LOD) of 1 mu g/L in urine and plasma. The recoveries were between 88 % and 111 % with the inter-day precision less than 13 %. An exposure study in 12 rats was conducted to determine urinary excretion of gyromitrin and its metabolite monomethylhydrazine (MMH). The mean urinary excretion rates of gyromitrin were 3.45 % (low dose) and 4.93 % (high dose), while the excretion rate of MMH were 0.20 % and 0.36 %. After exposure for 48 h, no gyromitrin and MMH was detected in rat urine. The results suggested that gyromitrin is a valuable biomarker for identification of gyromitrin-induced mushroom poisoning.

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