Efficient Constitutive Expression of Cellulolytic Enzymes in Penicillium oxalicum for Improved Efficiency of Lignocellulose Degradation

文献类型： 外文期刊

第一作者： Waghmare, Pankajkumar Ramdas

作者： Waghmare, Pankajkumar Ramdas;Waghmare, Pratima Pankajkumar;Gao, Liwei;Sun, Wan;Qin, Yuqi;Liu, Guodong;Qu, Yinbo;Gao, Liwei;Sun, Wan;Liu, Guodong;Sun, Wan;Qin, Yuqi;Liu, Guodong;Qu, Yinbo

作者机构：

关键词： Penicillium oxalicum; lignocellulose; constitutive expression system; cellulase; enzymatic conversion

期刊名称：JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：2.351； 五年影响因子：2.65 ）

ISSN： 1017-7825

年卷期： 2021 年 31 卷 5 期

页码：

收录情况： SCI

摘要： Efficient cellulolytic enzyme production is important for the development of lignocellulosedegrading enzyme mixtures. However, purification of cellulases from their native hosts is time-and labor-consuming. In this study, a constitutive expression system was developed in Penicillium oxalicum for the secreted production of proteins. Using a constitutive polyubiquitin gene promoter and cultivating with glucose as the sole carbon source, nine cellulolytic enzymes of different origins with relatively high purity were produced within 48 h. When supplemented to a commercial cellulase preparation, cellobiohydrolase I from P. funiculosum and cellobiohydrolase II from Talaromyces verruculosus showed remarkable enhancing effects on the hydrolysis of steam-exploded corn stover. Additionally, a synergistic effect was observed for these two cellobiohydrolases during the hydrolysis. Taken together, the constitutive expression system provides a convenient tool for the production of cellulolytic enzymes, which is expected to be useful in the development of highly efficient lignocellulose-degrading enzyme mixtures.

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