Immunological evaluation of recombination PRRSV GP3 and GP5 DNA vaccines in vivo

文献类型： 外文期刊

第一作者： Zhao, Guanyu

作者： Zhao, Guanyu;Zhang, Ying;Jin, Ningyi;Zhang, Jiaqi;Sun, Wenchao;Xie, Changzhan;Zhang, He;Gao, Yan;Wen, Shubo;Ha, Zhuo;Nan, Fulong;Zhu, Xiangyu;Feng, Sheng;Cao, Xinyu;Jin, Ningyi;Lu, Huijun;Zhu, Yanzhu;Zhu, Yanzhu

作者机构：

关键词： PRRSV; GP3; GP5; immune response; DNA vaccine; pig

期刊名称：FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY （ 影响因子：6.073； 五年影响因子：6.34 ）

ISSN： 2235-2988

年卷期： 2022 年 12 卷

页码：

收录情况： SCI

摘要： The porcine reproductive and respiratory syndrome virus (PRRSV) is a threat to the health of pigs worldwide, but commercially available vaccines offer limited protection against PRRSV infection. It is necessary to develop a more effective DNA vaccine. The immunological effects of DNA vaccines with three adjuvants were examined in pigs (Susscrofa domestica) challenged with PRRSV. These DNA vaccines, which encoded PRRSV GP3 and GP5, were formulated with A1, A2, and A3. Serum specific and neutralizing antibodies, IL-4, IFN-gamma, IL-2, IL-10, CD4(+) and CD8(+)T-lymphocytes, health status, histopathology, and viral loads were determined. The results showed that the use of adjuvant A3 led to higher levels of neutralizing antibodies and a lower viral load in pigs compared to the other adjuvants. The neutralizing antibody titers of the pVAX-GP35+A1 and pVAX-GP35+A3 groups reached a peak of 1:19 at 35 dpi. The maximum concentration of IL-4 was 136.77 pg/mL in the pVAX-GP35+A3 group. At 35 dpi, the IFN-gamma concentration in the pVAX-GP35+A1 group was 227.4 pg/mL. pVAX-GP35+A3 group shows the highest IL-2 and IL-10 expression to the peak of 597.6 pg/mL and 189.1 pg/mL, respectively. We found a formulation demonstrated beneficial immune outcomes. This study provides an alternative vaccine to protect pigs from PRRSV.

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