Isolation of expressed sequences from a specific chromosome of Thinopyrum intermedium infected by BYDV
文献类型: 外文期刊
第一作者: Yin, Wei-Bo
作者: Yin, Wei-Bo;Hu, Jun;Shi, Rui;Zhou, Ruo-Nan;Chen, Yu-Hong;Song, Li-Ying;Hu, Zan-Min;Shi, Rui;Zhou, Ruo-Nan;Song, Li-Ying;Zhou, Guang-He;Wang, Richard R. -C.
作者机构:
关键词: TAi-27;Thinopyrum intermedium;chromosome microdissection;hybridization-specific amplification (HSA);expressed sequences;barley yellow dwarf virus (BYDV)
期刊名称:GENOME ( 影响因子:2.166; 五年影响因子:2.474 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: To map important ESTs to specific chromosomes and (or) chromosomal regions is difficult in hexaploid wheat because of its large genome size and serious interference of homoeologous sequences. Large-scale EST sequencing and subsequent chromosome localization are both laborious and time-consuming. The wheat alien addition line TAi-27 contains a pair of chromosomes of Thinopyrum intermedium (Host) Barkworth & D.R. Dewey that carry the resistance gene against barley yellow dwarf virus. In this research, we developed a modified technique based on chromosome microdissection and hybridization-specific amplification to isolate expressed sequences from the alien chromosome of TAi-27 by hybridization between the DNA of the microdissected alien chromosome and cDNA of Th. intermedium infected by barley yellow dwarf virus. Twelve clones were selected, sequenced, and analyzed. Three of them were unknown genes without any hit in the GenBank database and the other nine were highly homologous with ESTs of wheat, barley, and (or) other plants in Gramineae induced by abiotic or biotic stress. The method used in this research to isolate expressed sequences from a specific chromosome has the following advantages: (i) the obtained expressed sequences are larger in size and have 3' end information and (ii) the operation is less complicated. It would be an efficient improved method for genomics and functional genomics research of polyploid plants, especially for EST development and mapping. The obtained expressed sequence data are also informative in understanding the resistance genes on the alien chromosome of TAi-27
分类号: Q3
- 相关文献
作者其他论文 更多>>
-
An automated lightweight approach for detecting dead fish in a recirculating aquaculture system
作者:Zhou, Chengquan;Wang, Chenye;Sun, Dawei;Hu, Jun;Ye, Hongbao;Wang, Chenye
关键词:YOLO; Dead fish; RAS; Image processing; Lightweight framework
-
Phylogenetic and Expression Analysis of the Sucrose Synthase and Sucrose Phosphate Synthase Gene Family in Potatoes
作者:Hu, Jun;Duan, Yanfeng;Li, Guangcun;Hu, Jun;Duan, Yanfeng;Li, Guangcun;Hu, Jinxue;Zhang, Shuqing
关键词:Solanum tuberosum L.; sucrose metabolism; gene expression; bioinformatics analysis
-
Mycorrhizal fungi reduce the photosystem damage caused by drought stress on Paris polyphylla var. yunnanensis
作者:Huang, Can;Zi, Shuhui;Xi, Congfang;Liu, Tao;Huang, Can;Zi, Shuhui;Liu, Tao;Huang, Can;He, Xiahong;Shi, Rui;Li, Xiaoxian
关键词:
-
Comparative Metabolome and Transcriptome Analyses Reveal Differential Enrichment of Metabolites with Age in Panax notoginseng Roots
作者:Yan, Xinru;Zhang, Ao;Jiao, Jinlong;He, Xiahong;Shi, Rui;Guan, Yiming;Zhang, Yayu;Ghanim, Murad
关键词:Panax notoginseng; gene expression; differential metabolites; therapeutic use
-
Transcriptome analysis of genes involved in the pathogenesis mechanism of potato virus Y in potato cultivar YouJin
作者:Yang, Tianqi;Zhao, Xingyue;Bai, Jinjiang;Zhou, Hongyou;Zhao, Mingmin;Zheng, Hongli;Lv, Wenxia;Hu, Jun;Liu, Guangjing;Chen, Qi;Zhao, Yuanzheng;Zhou, Hongyou;Zhao, Mingmin
关键词:potato virus Y; potato cultivar YouJin; RNA-Seq; functional analysis; candidate genes
-
Natural Glycyrrhizic Acid-Tailored Nanoparticles toward the Enhancement of Pesticide Bioavailability
作者:Wei, Kailun;Zheng, Zhiran;Hu, Jun;Li, Zilu;Gao, Yuxia;Huang, Qiliang;Li, Min-Hui
关键词:co-assembly; controlled-release; deposition; glycyrrhizic acid; nanoparticle
-
Identification of genetic loci for salt tolerance in Brassica napus at the germination stage using a genome-wide association study
作者:Yang, Tinghai;Zhu, Bin;Wang, Xiaodong;Yang, Tinghai;Zhou, Xiaoying;Cao, Yu;Shi, Rui;Wang, Yaping;Xiang, Yanan;Chen, Feng;Zhang, Wei;Sun, Chengming;Fu, Sanxiong;Hu, Maolong;Zhang, Jiefu;Wang, Xiaodong;Cao, Yu;Chen, Feng;Fu, Sanxiong;Hu, Maolong;Zhang, Jiefu;Wang, Xiaodong
关键词:Brassica napus; Genome-wide association study; Salt tolerance; SNP molecular marker; Candidate genes; Germplasm resources