Apoptotic and autophagic cell death induced in cervical cancer cells by a dual specific oncolytic adenovirus

文献类型： 外文期刊

第一作者： Li, Shanzhi

作者： Li, Shanzhi;Li, Zhuoxin;Zhu, Yilong;Li, Yiquan;Li, Xiao;Zhu, Guangze;Chen, Shuang;Li, Xiao;Yin, Xunzhe;Yin, Xunzhe

作者机构：

关键词： apoptosis; autophagy; cervical cancer HeLa cell lines; oncolytic adenovirus; RNAi

期刊名称：ANTI-CANCER DRUGS （ 影响因子：2.3； 五年影响因子：2.3 ）

ISSN： 0959-4973

年卷期： 2023 年 34 卷 3 期

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收录情况： SCI

摘要： ObjectiveOncolytic adenoviruses are capable of exerting anticancer effects via a variety of mechanisms, including apoptosis and autophagy. In the present study, the dual-specific antitumor oncolytic adenovirus, Ad-Apoptin-hTERT-E1a (ATV), was used to infect cervical cancer cell lines to test its antitumor effects. MethodsTo explore the use of apoptin in tumor gene therapy, a recombinant adenovirus ATV expressing the apoptin protein was assessed to determine its lethal and growth-inhibitory effects on human cervical cancer cell line (HeLa) cells in vitro. Nonapoptotic autophagy of HeLa cells infected with ATV was assessed by examining the cell morphology, development of acidic vesicular organelles and the conversion of microtubule-associated protein 1 light chain 3 (LC3) from its cytoplasmic to autophagosomal membrane form. Using gene silencing (knockdown of LC3 and Belin-1), autophagy-associated molecules (e.g. ATG5, ATG12 and ULK1) were monitored by real-time PCR and western blot. ResultsA series of experiments demonstrated that ATV could significantly induce apoptosis and autophagy in cervical cancer cells, and provided evidence that ATV not only induced apoptosis but also autophagy and ATG5, ATG12 and ULK1 related pathways were not entirely dependent on LC3 and Beclin-1. ConclusionThese results indicate that ATV may have a potential application in tumor gene therapy.

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