Pseudouridylation of chloroplast ribosomal RNA contributes to low temperature acclimation in rice

文献类型： 外文期刊

第一作者： Wang, Zhen

作者： Wang, Zhen;Sun, Jing;Zu, Xiaofeng;Gong, Jie;Deng, Hongjing;Hang, Runlai;Zhang, Xiaofan;Liu, Chunyan;Deng, Xian;Luo, Lilan;Song, Xianwei;Cao, Xiaofeng;Wang, Zhen;Sun, Jing;Zu, Xiaofeng;Gong, Jie;Deng, Hongjing;Hang, Runlai;Zhang, Xiaofan;Liu, Chunyan;Deng, Xian;Luo, Lilan;Song, Xianwei;Cao, Xiaofeng;Wang, Zhen;Zhang, Xiaofan;Cao, Xiaofeng;Gong, Jie;Wei, Xiangjin

作者机构：

关键词： chloroplast; pseudouridine synthase; reactive oxygen species (ROS); ribosome; rice; temperature

期刊名称：NEW PHYTOLOGIST （ 影响因子：10.323； 五年影响因子：10.768 ）

ISSN： 0028-646X

年卷期： 2022 年 236 卷 5 期

页码：

收录情况： SCI

摘要： Ribosomal RNAs (rRNAs) undergo many modifications during transcription and maturation; homeostasis of rRNA modifications is essential for chloroplast biogenesis in plants. The chloroplast acts as a hub to sense environmental signals, such as cold temperature. However, how RNA modifications contribute to low temperature responses remains unknown. Here we reveal that pseudouridine (psi) modification of rice chloroplast rRNAs mediated by the pseudouridine synthase (OsPUS1) contributes to cold tolerance at seedling stage. Loss-function of OsPUS1 leads to abnormal chloroplast development and albino seedling phenotype at low temperature. We find that OsPUS1 is accumulated upon cold and binds to chloroplast precursor rRNAs (pre-rRNAs) to catalyse the pseudouridylation on rRNA. These modifications on chloroplast rRNAs could be required for their processing, as the reduction of mature chloroplast rRNAs and accumulation of pre-rRNAs are observed in ospus1-1 at low temperature. Therefore, the ribosome activity and translation in chloroplasts is disturbed in ospus1-1. Furthermore, transcriptome and translatome analysis reveals that OsPUS1 balances growth and stress-responsive state, preventing excess reactive oxygen species accumulation. Taken together, our findings unveil a crucial function of psi in chloroplast ribosome biogenesis and cold tolerance in rice, with potential applications in crop improvement.

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