Genome-Wide Association Studies of Embryogenic Callus Induction Rate in Peanut (Arachis hypogaea L.)

文献类型： 外文期刊

第一作者： Luo, Dandan

作者： Luo, Dandan;Qu, Pengyu;Fu, Liuyang;Luo, Dandan;Shi, Lei;Sun, Ziqi;Qi, Feiyan;Liu, Hongfei;Xue, Lulu;Li, Xiaona;Liu, Han;Qu, Pengyu;Zhao, Huanhuan;Dai, Xiaodong;Dong, Wenzhao;Zheng, Zheng;Huang, Bingyan;Zhang, Xinyou;Shi, Lei;Sun, Ziqi;Qi, Feiyan;Dai, Xiaodong;Dong, Wenzhao;Zheng, Zheng;Huang, Bingyan;Zhang, Xinyou;Shi, Lei;Sun, Ziqi;Qi, Feiyan;Dai, Xiaodong;Dong, Wenzhao;Zheng, Zheng;Huang, Bingyan;Zhang, Xinyou;Shi, Lei;Sun, Ziqi;Qi, Feiyan;Zhang, Xinyou;Liu, Hongfei;Li, Xiaona;Liu, Han;Zhang, Xinyou

作者机构：

关键词： peanut; CIR; GWAS; candidate gene

期刊名称：GENES （ 影响因子：3.5； 五年影响因子：3.9 ）

ISSN：

年卷期： 2024 年 15 卷 2 期

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收录情况： SCI

摘要： The capability of embryogenic callus induction is a prerequisite for in vitro plant regeneration. However, embryogenic callus induction is strongly genotype-dependent, thus hindering the development of in vitro plant genetic engineering technology. In this study, to examine the genetic variation in embryogenic callus induction rate (CIR) in peanut (Arachis hypogaea L.) at the seventh, eighth, and ninth subcultures (T7, T8, and T9, respectively), we performed genome-wide association studies (GWAS) for CIR in a population of 353 peanut accessions. The coefficient of variation of CIR among the genotypes was high in the T7, T8, and T9 subcultures (33.06%, 34.18%, and 35.54%, respectively), and the average CIR ranged from 1.58 to 1.66. A total of 53 significant single-nucleotide polymorphisms (SNPs) were detected (based on the threshold value -log(10)(p) = 4.5). Among these SNPs, SNPB03-83801701 showed high phenotypic variance and neared a gene that encodes a peroxisomal ABC transporter 1. SNPA05-94095749, representing a nonsynonymous mutation, was located in the Arahy.MIX90M locus (encoding an auxin response factor 19 protein) at T8, which was associated with callus formation. These results provide guidance for future elucidation of the regulatory mechanism of embryogenic callus induction in peanut.

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