Metabolomics and proteomics reveal the molecular basis of colour formation in the pericarp of Chinese wild rice (Zizania latifolia)

文献类型： 外文期刊

第一作者： Yu, Xiuting

作者： Yu, Xiuting;Qi, Qianqian;Li, Yali;Xie, Yanning;Ding, Anming;Du, Yongmei;Liu, Xinmin;Zhang, Zhongfeng;Yan, Ning;Yu, Xiuting;Qi, Qianqian;Li, Yali;Li, Nana;Shi, John;Ding, Anming;Yan, Ning

作者机构：

关键词： Chinese wild rice(Zizanialatifolia); Colour formation; Flavonoids; Metabolomics; Proteomics

期刊名称：FOOD RESEARCH INTERNATIONAL （ 影响因子：7.425； 五年影响因子：7.716 ）

ISSN： 0963-9969

年卷期： 2022 年 162 卷

页码：

收录情况： SCI

摘要： Chinese wild rice (Zizania latifolia) is rich in flavonoids and the characteristic colour of its pericarp is attributed to the flavonoids. In this study, the molecular basis of the colour change in the pericarp of Chinese wild rice was studied using metabolomics and proteomics. Whole seeds in three developmental stages (10, 20, and 30 days after flowering) were characterised based on phenolic contents, free amino acids (FAAs), and the expression level and activities of enzymes critical in flavonoid biosynthesis. The total phenolic and proanthocyanidin contents of Chinese wild rice increased gradually, whereas total flavonoid and FAA contents decreased during seed devel-opment. Metabolomic analysis revealed gradual upward trends for 57 flavonoids (sub classes 1, 3, and 10) related to colour change in the pericarp. Proteomic analysis showed that the phenylpropanoid biosynthesis metabolic pathway was enriched with differentially expressed proteins and was associated with flavonoid biosynthesis. Proteomic data suggested that leucoanthocyanidin reductase and WD40 repeat protein may be involved in flavonoid biosynthesis in Chinese wild rice, which was also verified by real-time quantitative PCR. Our results provide new insights into the understanding of the colour formation in the pericarp of Chinese wild rice.

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