Development and characterization of 123 SNPs in an endangered fish Semilabeo obscurus

文献类型: 外文期刊

第一作者: Li, Shikai

作者: Li, Shikai;Wang, Wei;Yang, Mingju;Huang, Fujiang;Wang, Xue;Zeng, Sheng;Xiang, Yan;Zhou, Lu;Li, Shikai;Wang, Wei;Yang, Mingju;Huang, Fujiang;Wang, Xue;Zeng, Sheng;Xiang, Yan;Zhou, Lu

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关键词: Semilabeo obscurus; SNP; Molecular marker; Germplasm conservation

期刊名称:CONSERVATION GENETICS RESOURCES ( 影响因子:0.991; 五年影响因子:0.897 )

ISSN: 1877-7252

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收录情况: SCI

摘要: Semilabeo obscurus, as an endangered species, is a certain economic fish in China and Vietnam. However, study on its genetic diversity is still lacking. In this study, 168 candidate SNPs were verified by Sanger-sequencing. Of these, 123 polymorphic SNP markers were successfully genotyped. The observed heterozygosity (Ho), expected heterozygosity (He) and polymorphic information conten (PIC) ranged from 0.0968-0.9310 (mean 0.3590), 0.0936-0.5085 (mean 0.3550) and 0.0879-0.3750 (mean 0.2807), respectively. Only 3 SNPs (Sob-016, Sob-050 and Sob-115) were significantly deviated from Hardy-Weinberg equilibrium (P < 0.05). Our study may help researchers better understand the genetic traits of S. obscurus,and provide reference for further protecting and reasonably utilizing its genetic resources.

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