A Comparative Transcriptome and Metabolome Combined Analysis Reveals the Key Genes and Their Regulatory Model Responsible for Glucoraphasatin Accumulation in Radish Fleshy Taproots

文献类型： 外文期刊

第一作者： Li, Xiaoman

作者： Li, Xiaoman;Liu, Liwang;Li, Xiaoman;Wang, Peng;Wang, Jinglei;Wang, Haiping;Liu, Tongjin;Zhang, Xiaohui;Song, Jiangping;Yang, Wenlong;Wu, Chunhui;Yang, Haohui;Li, Xixiang;Wang, Jinglei;Liu, Tongjin

作者机构：

关键词： glucosinolate profiles; RNA-seq; RsMYB28; key genes; regulatory network; radish fleshy taproots; genotypes

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）

ISSN：

年卷期： 2022 年 23 卷 6 期

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收录情况： SCI

摘要： Radish (Raphanus sativus L.) is rich in specific glucosinolates (GSLs), which benefit human health and special flavor formation. Although the basic GSLs metabolic pathway in Brassicaceae plants is clear, the regulating mechanism for specific glucosinolates content in radish fleshy taproots is not well understood. In this study, we discovered that there was a significant difference in the GSLs profiles and the content of various GSLs components. Glucoraphasatin (GRH) is the most predominant GSL in radish taproots of different genotypes as assessed by HPLC analysis. Further, we compared the taproot transcriptomes of three radish genotypes with high and low GSLs content by employing RNA-seq. Totally, we identified forty-one differentially expressed genes related to GSLs metabolism. Among them, thirteen genes (RsBCAT4, RsIPMDH1, RsMAM1a, RsMAM1b, RsCYP79F1, RsGSTF9, RsGGP1, RsSUR1, RsUGT74C1, RsST5b, RsAPK1, RsGSL-OH, and RsMYB28) were significantly higher co-expressed in the high content genotypes than in low content genotype. Notably, correlation analysis indicated that the expression level of RsMYB28, as an R2R3 transcription factor directly regulating aliphatic glucosinolate biosynthesis, was positively correlated with the GRH content. Co-expression network showed that RsMYB28 probably positively regulated the expression of the above genes, particularly RsSUR1, and consequently the synthesis of GRH. Moreover, the molecular mechanism of the accumulation of this 4-carbon (4C) GSL in radish taproots was explored. This study provides new perspectives on the GSLs accumulation mechanism and genetic improvements in radish taproots.

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[1]A Comparative Transcriptome and Metabolome Combined Analysis Reveals the Key Genes and Their Regulatory Model Responsible for Glucoraphasatin Accumulation in Radish Fleshy Taproots. Li, Xiaoman,Liu, Liwang,Li, Xiaoman,Wang, Peng,Wang, Jinglei,Wang, Haiping,Liu, Tongjin,Zhang, Xiaohui,Song, Jiangping,Yang, Wenlong,Wu, Chunhui,Yang, Haohui,Li, Xixiang,Wang, Jinglei,Liu, Tongjin. 2022

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