Identification of a major QTL and genome-wide epistatic interactions for single vs. paired spikelets in a maize-teosinte F-2 population

文献类型： 外文期刊

第一作者： Chen, Zhengjie

作者： Chen, Zhengjie;Hu, Kun;Yin, Yong;Tang, Dengguo;Ni, Jixing;Li, Peng;Wang, Le;Rong, Tingzhao;Liu, Jian;Chen, Zhengjie

作者机构：

关键词： Maize; Teosinte; Single spikelet; Paired spikelets; Major QTL; Epistasis; Segregation distortion region

期刊名称：MOLECULAR BREEDING （ 影响因子：3.297； 五年影响因子：3.011 ）

ISSN： 1380-3743

年卷期： 2022 年 42 卷 2 期

页码：

收录情况： SCI

摘要： Maize ear carries paired spikelets, whereas the ear of its wild ancestor, teosinte, bears single spikelets. However, little is known about the genetic basis of the processes of transformation of single spikelets in teosinte ear to paired spikelets in maize ear. In this study, a two-ranked, paired-spikelets primitive maize and a two-ranked, single-spikelet teosinte were utilized to develop an F-2 population, and quantitative trait locus (loci) (QTL) mapping for single vs. paired spikelets (PEDS) was performed. One major QTL (qPEDS3.1) for PEDS located on chromosome 3S was identified in the 162 F-2 plants using the inclusive composite interval mapping of additive (ICIM-ADD) module, explaining 23.79% of the phenotypic variance. Out of the 409 F-2 plants, 43 plants with PEDS = 0% and 43 plants with PEDS > 20% were selected for selective genotyping, and the QTL (qPEDS3.1) was detected again. Moreover, the QTL (qPEDS3.1) was validated in three environments, which explained 31.05%, 38.94%, and 23.16% of the phenotypic variance, respectively. In addition, 50 epistatic QTLs were detected in the 162 F-2 plants using the two-locus epistatic QTL (ICIM-EPI) module; they were distributed on all 10 chromosomes and explained 94.40% of the total phenotypic variance. The results contribute to a better understanding of the genetic basis of domestication of paired spikelets and provide a genetic resource for future map-based cloning; in addition, the systematic dissection of epistatic interactions underlies a theoretical framework for overcoming epistatic effects on QTL fine mapping.

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