Application and potential therapeutic effect of endolysin Lys1472 against Clostridium perfringens in chicken meat

文献类型： 外文期刊

第一作者： Hou, Bingyu

作者： Hou, Bingyu;Zhang, Mengrui;Cai, Yumei;Zhang, Mengrui;Lv, Qianghua;Tang, Zhaohui;Zhang, Qing;Liu, Yuqing;Zhao, Xiaonan;Lv, Qianghua;Tang, Zhaohui;Zhang, Qing;Liu, Yuqing;Zhao, Xiaonan;Zhang, Qing

作者机构：

关键词： Clostridium perfringens; Endolysin; Lytic activity; Chicken; Food safety

期刊名称：LWT-FOOD SCIENCE AND TECHNOLOGY （ 影响因子：6.6； 五年影响因子：6.9 ）

ISSN： 0023-6438

年卷期： 2025 年 216 卷

页码：

收录情况： SCI

摘要： Clostridium perfringens is a well-known pathogen that induces foodborne illness worldwide. In this study, the presumed endolysin gene Lys1472 from C. perfringens 2-2 was cloned and expressed in E. coli. Lys1472 has a catalytic domain homologous to that of GH25_muramidase and two SH3B binding domains. Lys1472 displayed excellent lytic activity against C. perfringens with a lytic rate of 100% and was stable in optimum conditions at temperatures (-20 similar to 50 degrees C), pH ranges (4-10), and NaCl (0-500 mM). All the tested metal ions slightly decreased the lytic activity of Lys1472, which was completely inactivated under divalent conditions of Zn2+ and Fe2+. Lys1472 treatment improved the survival rate of the larvae to 85% at 24 h Lys1472 treatment inhibited C. perfringens growth on chicken breast, yielding a 3-log reduction at 0, 3, 6 and 12 h, at 4 degrees C. Lys1472 treatment inhibited C. perfringens growth on chicken breast, resulting in a 3-log reduction at 0, 3, and 6 h, at 25 degrees C. The results of this study suggest that Lys1472 could serve as an alternative for controlling and treating foodborne C. perfringens contamination.

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