Transposon-tagging identifies novel pathogenicity genes in Fusarium graminearum

文献类型： 外文期刊

第一作者： Ben M'Barek, Sarrah

作者： Ben M'Barek, Sarrah;Liu, Taiguo;Waalwijk, Cees;Daboussi, Marie-Josee;van der Lee, Theo;Ben M'Barek, Sarrah;Xu, Xiude;Zhang, Xu;Zhang, Wenwei;Kema, Gert H. J.;Xu, Xiude;Zhang, Xu;Liu, Taiguo;Zhang, Wenwei

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期刊名称：FUNGAL GENETICS AND BIOLOGY （ 影响因子：3.495； 五年影响因子：3.831 ）

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收录情况： SCI

摘要： With the increase of sequenced fungal genomes, high-throughput methods for functional analyses of genes are needed. We assessed the potential of a new transposon mutagenesis tool deploying a Fusarium oxysporum miniature inverted-repeat transposable element mimp1, mobilized by the transposase of impala, a Tc1-like transposon, to obtain knock-out mutants in Fusarium graminearum. We localized 91 mimp1 insertions which showed good distribution over the entire genome. The main exception was a major hotspot on chromosome 2 where independent insertions occurred at exactly the same nucleotide position. Furthermore insertions in promoter regions were over-represented. Screening 331 mutants for sexual development, radial growth and pathogenicity on wheat resulted in 19 mutants (5.7%) with altered phenotypes. Complementation with the original gene restored the wild-type phenotype in two selected mutants demonstrating the high tagging efficiency. This is the first report of a MITE transposon tagging system as an efficient mutagenesis tool in F. graminearum.

分类号： Q939.5

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