Novel IGF2BP1 splice variants, expression and their association with growth traits in goose
文献类型: 外文期刊
第一作者: Wang, C.
作者: Wang, C.;Yang, Y.;Liu, Y.;Li, G.;Wang, X.;Wang, H.;Gong, S.;He, D.;Zhang, Y.;Chen, S.;Song, J.;Lin, Z.
作者机构:
关键词: Geese; IGF2BP1 gene expression; correlation; body weight
期刊名称:BRITISH POULTRY SCIENCE ( 影响因子:1.892; 五年影响因子:2.429 )
ISSN: 0007-1668
年卷期:
页码:
收录情况: SCI
摘要: 1. The gene IGF2BP1 has been reported as being related to the body size and body weight in ducks, goats and chickens. However, this potential growth-related gene has not yet been reported in geese. 2. The goose IGF2BP1 cDNA (IGF2BP1-X1) is 2,925 bp in length, containing an open reading frame (ORF) of 1,731 bp that encodes a protein of 576 amino acids. Six IGF2BP1 transcripts (IGF2BP1-X2 similar to IGF2BP1-X7) were identified due to the alternative splicing of different exons within the IGF2BP1-X1 transcript. 3. RT-qPCR analysis indicated that the goose IGF28P1 mRNA was differentially expressed in the examined tissues of female embryos (28 d of development), adult (70 d of age) and laying (270 d of age) Zhedong White geese. 4. 4. In total, 16 single nucleotide polymorphisms (SNPs) and three insertion/deletion (InDel) variants were detected in several introns and 3'-untranslated regions of the goose IGF2BP1 gene. The 17-bp InDel within IGF2BP1 intron 14 was significantly associated with body weight at six weeks old (BW6, P < 0.05), and extremely significantly associated with the BW8 and BW10 (P < 0.01) of Zhedong White geese.
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