Diversities of African swine fever virus host-virus dynamics revealed by single-cell profiling

文献类型： 外文期刊

第一作者： Zhao, Xiaoyang

作者： Zhao, Xiaoyang;Lv, Lin;Ahsan, Md. Asif;Fu, Xudong;Shen, Ning;Zhao, Xiaoyang;Lv, Lin;Ahsan, Md. Asif;Fu, Xudong;Shen, Ning;Zhang, Yanyan;Hu, Rongliang;Jia, Hanying;Shen, Zhiqiang;Shen, Zhiqiang

作者机构：

关键词： single-cell RNA sequencing; African swine fever virus; host-virus interaction; macrophage

期刊名称：JOURNAL OF VIROLOGY （ 影响因子：3.8； 五年影响因子：3.9 ）

ISSN： 0022-538X

年卷期： 2025 年 99 卷 3 期

页码：

收录情况： SCI

摘要： African swine fever virus (ASFV) causes epidemics with high mortality; however, effective vaccines and therapies remain missing. Here, we depict a temporal single-cell landscape of primary porcine alveolar macrophages (PAMs) exposed to three different virulent ASFV strains in vitro. We found that attenuated and low-virulence ASFV strains tend to exhibit higher viral loads than highly virulent strain, which may result from upregulated RNA polymerase subunit genes expression. On the host side, our study highlights the IRF7-mediated positive feedback loop to the activation of the interferon signaling pathway in cells exposed to attenuated and low virulent ASFV strains. Moreover, we unraveled the PAMs populations marked by expressions of the IFI16 and CD163, respectively, which produce high levels of interferon-stimulated genes (ISGs) and IL18 to regulate the host response to different virulent ASFV strains. Collectively, our data provide insights into the complex host-virus interactions with various ASFV strain infections, which may shed light on the development of effective antiviral strategies.

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