EXTRACTION AND ISOLATION OF DICTAMNINE, OBACUNONE AND FRAXINELLONE FROM Dictamnus dasycarpus TURCZ. BY SUPERCRITICAL FLUID EXTRACTION AND HIGH-SPEED COUNTER-CURRENT CHROMATOGRAPHY

文献类型： 外文期刊

第一作者： Wang, Daijie

作者： Wang, Daijie;Lin, Yunliang;Lin, Xiaojing;Geng, Yanling;Wang, Xiao;Zhang, Jinjie;Qiu, Jiying

作者机构：

关键词： Dictamnus dasycarpus; dictamnine; obacunone

期刊名称：QUIMICA NOVA （ 影响因子：0.961； 五年影响因子：1.091 ）

ISSN： 0100-4042

年卷期： 2012 年 35 卷 2 期

页码：

收录情况： SCI

摘要： Supercritical fluid extraction was used to extract active compounds from the Chinese traditional medicinal D. dasycarpus under the pressure of 30 MPa and temperature of 45 degrees C. Further separation and purification was established by high-speed counter-current chromatography (HSCCC) with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (1:0.8:1.3:0.9, volume ratio). The separation yielded a total of 47 mg of dictamnine, 24 mg of obacunone and 83 mg of fraxinellone from 1.0 g of the crude extract in one step separation with the purity of 99.2, 98.4 and 99.0%, respectively, as determined by HPLC. The chemical structures of these compounds were identified by ES1-MS, IR, H-1-NMR and C-13-NMR.

分类号： O6

  • 相关文献
作者其他论文 更多>>

微信小程序