Antioxidant Activity and the Potential Mechanism of the Fruit From Ailanthus altissima Swingle

文献类型： 外文期刊

第一作者： Mo, Ya-nan

作者： Mo, Ya-nan;Cheng, Feng;Yang, Zhen;Shang, Xiao-fei;Liang, Jian-ping;Shang, Ruo-feng;Hao, Bao-cheng;Wang, Xue-hong;Zhang, Hong-juan;Liu, Yu;Cheng, Feng;Wali, Ahmidin;Lu, Chun-fang;Liu, Yu

作者机构：

关键词： Ailanthus altissima Swingle; antioxidant activity; RAW264; 7 cell; network pharmacology; in vitro; antioxidant mechanism

期刊名称：FRONTIERS IN VETERINARY SCIENCE （ 影响因子：3.471； 五年影响因子：3.821 ）

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年卷期： 2021 年 8 卷

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收录情况： SCI

摘要： The fruits of Ailanthus altissima Swingle (AS) possess a variety of pharmacological activities. Its antioxidant activity and the potential mode of action have not yet been investigated. In in vitro studies, AS revealed the strong reducing power and DPPH scavenging effect, but hydroxyl radical scavenging activity and ferrous ions-chelating ability were not strong. Meanwhile, the oxidative stress RAW264.7 cell injury model was established, the low and medium-doses of AS showed significant protective effects on the viability of H2O2-treated cells by CCK-8 method. Besides, three doses of AS all increased the activities of SOD, CAT, and GSH-Px and decreased the MDA level compared with the H2O2 group, suggesting it significantly relieved oxidative stress of cells. The active ingredients and related targets of AS were collected by HERB and Swiss Target Prediction database, the common targets of drugs and diseases database were conducted by GeneCards database platform and the Venny platform. We screened the core targets of AS like threonine kinase1 (AKT1), mitogen-activated protein kinase 1 (MAPK1), sirtuin-1 (SIRT1), mechanistic target of rapamycin kinase (MTOR) by STRING database, and the key pathways involved PI3K-AKT and FoxO signaling pathway by KEGG pathway enrichment analysis. Besides, qRT-PCR revealed AS preconditioning significantly up-regulated the expression level of AKT1, SIRT1, MAPK1, and MTOR in model cells, and the effect was related to the regulation of FoxO and PI3K/AKT signaling pathway. In summary, AS showed significant antioxidant activity and its potential mechanism was regulating FoxO and PI3K/AKT signaling pathway.

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