Purification and characterization of a novel protease-resistant alpha-galactosidase from Rhizopus sp. F78 ACCC 30795
文献类型: 外文期刊
第一作者: Cao, Yanan
作者: Cao, Yanan;Yang, Peilong;Shi, Pengjun;Wang, Yaru;Luo, Huiying;Meng, Kun;Zhang, Zhifang;Wu, Ningfeng;Yao, Bin;Fan, Yunliu
作者机构:
关键词: Alpha-galactosidase;Rhizopus sp.;Characterization;Protease resistant
期刊名称:ENZYME AND MICROBIAL TECHNOLOGY ( 影响因子:3.493; 五年影响因子:3.699 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: A novel extracellular a-galactosidase, named Aga-F78, from Rhizopus sp. F78 ACCC 30795 was induced, purified and characterized in this study. This soybean-inducible a-galactosidase was purified to homogeneity by ammonium sulfate precipitation and fast protein liquid chromatography (FPLC), with a yield of 14.6% and a final specific activity of 74.6 U mg~(-1). Aga-F78 has an estimated relative molecular mass of 78 kDa from SDS-PAGE while native mass of 210 kDa and 480 kDa from non-denaturing gradient PAGE. This alpha-galactosidase had no N- or O-glycosylated. Amino acid sequences of three internal fragments were determined, and fragment 1, NQLVLDLTR, shared high homology with bacterial and fungal GH-36 alpha-galactosidases. The optimum pH and temperature on activity of Aga-F78 were 4.8 and 50 deg C, respectively. The properties of pH and temperature stability, effect of ions and chemicals were also studied. Furthermore, the resistant to neutral and alkaline proteases and substrate specificity of natural substrates (melibiose, raffinose, stachyose and guar gum) were also studied to enlarged the application of Aga-F78 in more fields. Kinetic studies revealed a K_m and V_(max) of 2.9 mmol l~(-1) and 246.1 mu mol (mg min)~(-1), respectively, using pNPG as substrate. To our knowledge, this is the first report of purification and characterization of alpha-galactosidase from Rhizopus with some special properties, which may aid its utilization in the food and feed industries.
分类号: Q55
- 相关文献
作者其他论文 更多>>
-
Fluorescent Probes Based on Ag NPs@N/GQDs and Molecularly Imprinted Polymer for Sensitive Detection of Noradrenaline in Bananas
作者:Wang, Yaru;Wang, Mingyue;Liu, Sixin;Wang, Yaru;Li, Shuhuai;Ma, Xionghui;Pang, Chaohai;Wu, Yuwei;Wang, Mingyue;Wang, Yaru;Li, Shuhuai;Wang, Mingyue;Li, Bei
关键词:Fluorescent probe; Graphene quantum dots; Silver nanoparticles; Molecular imprinted polymer; Noradrenaline
-
High-Throughput Screening Techniques for the Selection of Thermostable Enzymes
作者:Li, Lanxue;Bai, Yingguo;Yao, Bin;Luo, Huiying;Tu, Tao;Liu, Xiaoqing
关键词:high-throughput screening; virtual screening; protein engineering; enzyme thermostability
-
A SARS-CoV-2 Nanobody Displayed on the Surface of Human Ferritin with High Neutralization Activity
作者:Zhang, Wenrong;Wang, Haining;Wu, Tong;Gao, Xintao;Shang, Yuting;Zhang, Zhifang;Liu, Xingjian;Li, Yinu;Zhang, Wenrong
关键词:COVID-19; ferritin; fenobody; baculovirus expression system; silkworm
-
Recombinant production of SAG1 fused with xylanase in Pichia pastoris induced higher protective immunity against Eimeria tenella infection in chicken
作者:Liu, Chen;Wei, HanBing;Wang, Yuan;Su, Xiaoyun;Tu, Tao;Luo, Huiying;Yao, Bin;Huang, Huoqing;Zhang, Honglian;Liang, Ruiying;Ding, Jiabo;Tang, Xinming
关键词:
-
Characterization of a Novel Hyperthermophilic GH1 β-Glucosidase from Acidilobus sp. and Its Application in the Hydrolysis of Soybean Isoflavone Glycosides
作者:He, Jinjian;Li, Yuying;Sun, Xihang;Zuo, Dinghui;Wang, Mansheng;Zheng, Xia;Shi, Pengjun;Zuo, Dinghui;Yu, Pinglian
关键词:beta-glucosidase; Acidilobus sp.; thermophilic; thermostability; ethanol tolerance; isoflavone glycosides
-
Puerariae Radix protects against ulcerative colitis in mice by inhibiting NLRP3 inflammasome activation
作者:Ga, Yu;Wei, Yuanyuan;Zhao, Qingyu;Fan, Yimeng;Zhang, Yannan;Hao, Zhihui;Ga, Yu;Wei, Yuanyuan;Zhao, Qingyu;Fan, Yimeng;Zhang, Yannan;Hao, Zhihui;Zhang, Zhifang;Hao, Sijia;Wang, Lixia;Wang, Zhifen;Han, Jinlong;Wu, Shuang
关键词:Ulcerative colitis; Molecular mechanisms; Pyroptosis
-
Cooperative Fermentation Using Multiple Microorganisms and Enzymes Potentially Enhances the Nutritional Value of Spent Mushroom Substrate
作者:Zhang, Anrong;He, Weizhao;Han, Yunsheng;Zheng, Aijuan;Chen, Zhimin;Meng, Kun;Yang, Peilong;Liu, Guohua
关键词:fermentation; Pleurotus eryngii; nutritional value; condition optimization; enzymatic treatment