NUA4 histone acetylase gene LeEaf6 regulates precocity of shiitake mushroom, Lentinula edodes

文献类型： 外文期刊

第一作者： Wang, Lu

作者： Wang, Lu;Gong, Wenbing;Xie, Haoyu;Xiao, Yang;Gong, Wenbing;Liu, Gaolei

作者机构：

关键词： Shiitake mushroom; Precocity; Fruiting; Histone acetyltransferase

期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：4.3； 五年影响因子：4.5 ）

ISSN： 0304-4238

年卷期： 2024 年 328 卷

页码：

收录情况： SCI

摘要： Breeding excellent earlier-fruiting cultivars is of great concern in mushroom cultivation and breeding, as they could greatly reduce the costs during the commercial cultivation. Therefore, it is crucial to uncover the regulatory genes and their functions underlying mushroom precocity. Eaf6 is a subunit of NuA4 complex, a highly conserved histone acetyltransferase that functions in transcriptional regulation. In our previous studies, a Eaf6 homolog LeEaf6 was identified as a critical candidate gene regulating precocity in Lentinula edodes through quantitative trait loci mapping and genome-wide association study. Herein, we further cloned LeEaf6 and elucidated its function through overexpression and RNA interference in L. edodes. Sequence analysis indicated that LeEaf6 was 898 bp in length, encoding a stable protein with 111 amino acids. Subcellular localization showed that LeEaf6 protein was present in nucleus. By phenotypic evaluation and analysis, significant differences of precocity were revealed between LeEaf6 transformants and the control strain. The periods required for primordium formation and fruiting body maturity were 3-9 days and 6-12 days advanced in LeEaf6 overexpression transformants. In contrast, the periods of primordium formation and fruiting body maturation was delayed by 6-10 days and 9-12 days in LeEaf6-RNAi transformants. However, overexpression and interference of LeEaf6 had no significant effect on mycelial growth, fruiting body morphology, and yield-related traits in L. edodes. Collectively, LeEaf6 promoted precocity without affecting vegetative growth and other agronomic traits, and emerged as a promising gene target for breeding early-fruiting cultivars in L. edodes. To our knowledge, LeEaf6 was the first validated gene regulating precocity in mushrooms. Findings here could advance our understanding of genetic underpinnings of mushroom fruiting, and are beneficial for genetics and breeding of L. edodes.

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