Multi-omic analysis provides new insights into the regulatory mechanism of the LED-induced delay of postharvest senescence and yellowing in broccoli

文献类型： 外文期刊

第一作者： Wang, Zhiqiang

作者： Wang, Zhiqiang;Lv, Jieyu;Bin, Yuqi;Duan, Wenhui;Shi, Junyan;Gao, Hongdou;Yuan, Shuzhi;Yue, Xiaozhen;Zuo, Jinhua;Wang, Qing;Lv, Jieyu;Duan, Wenhui;Xu, Xiangbin;Wang, Zhiqiang;Li, Xiaojiao

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关键词： Broccoli; Red LED light irradiation; Postharvest; Gene expression; Oxidative stress

期刊名称：POSTHARVEST BIOLOGY AND TECHNOLOGY （ 影响因子：6.8； 五年影响因子：7.5 ）

ISSN： 0925-5214

年卷期： 2025 年 230 卷

页码：

收录情况： SCI

摘要： MicroRNAs (miRNAs) play a significant role in regulating plant growth, development, and senescence. However, the molecular mechanism through which miRNAs regulate senescence and yellowing of broccoli is unclear. Here, we report on an integrated analysis of transcriptome, small RNA-sequencing, and degradome sequencing data, which revealed that miRNAs mediate senescence and yellowing of broccoli by decreasing peroxidase activity, regulating chlorophyll catabolism, and maintaining ROS homeostasis. Red LED light irradiation induced the expression of miR9557-5p, miR160a-5p, miR169e-3p and miR9557-5p which in turn suppressed the expression of their target genes, BoPRX7, BoPRX17, BoPRX22, and BoPRX67, respectively. In addition, red LED irradiation suppressed the expression of miR159c-5p, miR168a-5p, PC-5P-98712, and miR393b-3p, which in turn enhanced the expression of their target genes, BoCHLH, BoPSAK, BoPORC, and BoCHLD, respectively. This regulatory model helps to maintain the dynamic balance between ROS production and ROS scavenging, the biosynthesis of chlorophyll, and ROS homeostasis, thereby delaying the senescence and yellowing of broccoli. Collectively, our results indicate that miRNAs regulate the yellowing and senescence of broccoli by regulating the expression of target genes related to peroxidase activity, chlorophyll-binding, and chlorophyll biosynthetic processes. these findings enhance our understanding of the regulatory mechanisms in broccoli controlling yellowing and senescence.

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