5 mC modification of steroid hormone biosynthesis-related genes orchestrates feminization of channel catfish induced by high-temperature

文献类型： 外文期刊

第一作者： Xu, Siqi

作者： Xu, Siqi;Xie, Bingjie;Liu, Hongyan;Liu, Ju;Wang, Minghua;Zhong, Liqiang;Chen, Xiaohui;Zhang, Shiyong;Xu, Siqi;Liu, Hongyan;Wang, Minghua;Zhong, Liqiang;Chen, Xiaohui;Zhang, Shiyong;Chen, Xiaohui;Zhang, Shiyong;Xie, Bingjie;Chen, Xiaohui;Zhou, Jian;Zhang, Lu;Wen, Zhengyong

作者机构： Natl Genet Breeding Ctr Channel Catfish, Freshwater Fisheries Res Inst Jiangsu Prov, Nanjing 210027, Peoples R China;Southwest Univ, Coll Fisheries, Integrat Sci Ctr Germplasm Creat Western China CHO, Chongqing 402460, Peoples R China;Jiangsu Prov Platform Conservat & Utilizat Agr Ger, Nanjing 210014, Peoples R China;Jiangsu Ocean Univ, Coll Marine Sci & Fisheries, Lianyungang 222005, Peoples R China;Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China;Sichuan Acad Agr Sci, Fisheries Res Inst, Chengdu 611731, Peoples R China;Neijiang Normal Univ, Key Lab Sichuan Prov Fishes Conservat & Utilizat U, Neijiang 641100, Peoples R China

关键词： Temperature; Sex reversal; Methylation; srd5a2; Channel catfish

期刊名称：ENVIRONMENTAL POLLUTION （ 2023影响因子：7.6； 五年影响因子：8.3 ）

ISSN： 0269-7491

年卷期： 2024 年 356 卷

页码：

收录情况： SCI

摘要： To elucidate the mechanism behind channel catfish feminization induced by high temperature, gonad samples were collected from XY pseudo -females and wild -type females and subjected to high -throughput sequencing for Whole-Genome-Bisulfite-Seq (WGBS) and transcriptome sequencing (RNA-Seq). The analysis revealed 50 differentially methylated genes between wild -type females and XY pseudo -females, identified through the analysis of KEGG pathways and GO enrichment in the promoter of the genome and differentially methylated regions (DMRs). Among these genes, multiple differential methylation sites observed within the srd5a2 gene. Repeatability tests confirmed 7 differential methylation sites in the srd5a2 gene in XY pseudo -females compared to normal males, with 1 specific differential methylation site (16608174) distinguishing XY pseudo -females from normal females. Interestingly, the expression of these genes in the transcriptome showed no difference between wild -type females and XY pseudo -females. Our study concluded that methylation of the srd5a2 gene sequence leads to decreased expression, which inhibits testosterone synthesis while promoting the synthesis of 17 beta- estradiol from testosterone. This underscores the significance of the srd5a2 gene in the sexual differentiation of channel catfish, as indicated by the ipu00140 KEGG pathway analysis.

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