Renaturation and purification of ApxII toxin of Actinobacillus pleuropneumoniae

文献类型： 外文期刊

第一作者： Wang, Chunlai

作者： Wang, Chunlai;Liu, Siguo;Peng, Yonggang;Shao, Meili;Wang, Yong;Gong, Qiang;Chang, Yuehong;Liu, Jiandong;Liu, Huifang;Liu, Di;Kong, Xiangang

作者机构：

关键词： expression;renaturation;purification;ApxII toxin;hemolytic activity;PORCINE CONTAGIOUS PLEUROPNEUMONIA;ESCHERICHIA-COLI;RTX-TOXINS;VIRULENCE;SEROTYPE-1;EXPRESSION;PIGS;HEMOLYSINS;SUBUNIT;OPERONS

期刊名称：PROTEIN EXPRESSION AND PURIFICATION （ 影响因子：1.65； 五年影响因子：1.548 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： ApxII toxin is the only Apx toxin that is produced by Actinobacillus pleuropneumoniae serotype 7. In order to determine whether the recombinant ApxII that derived from Escherichia coli (E. coli) expression is faithful to the natural ApxII so that can be used as additional component in vaccine preparation, the structure gene apxIIA of ApxII toxin was expressed in E coli with prokaryotic expression vector pGEX-6p-1 (formed pGEX-6p-A). pGZRS-C which is A. pleuropneunionicte-E. coli shuttle vector pGZRS-38 expressing the post-transcriptional activation gene apxII C was co-expressed with pGEX-6p-A. The expression product of rApxII A formed inclusion. The inclusion protein was oxidized, refolded and restored hemolytic activity after denaturation, renaturation and purification. The result indicated that E. coli expressed recombinant ApxII toxin has good fidelity, which makes it possible to produce this valuable antigen for vaccine preparation or diagnosis. (c) 2006 Elsevier Inc. All rights reserved.

分类号： Q51

  • 相关文献

[1]Eukaryotic expression and antimicrobial spectrum determination of the peptide tachyplesin II. Xu, Feng,Meng, Kun,Wang, Ya-Ru,Lu, Hui-Ying,Yang, Pei-Long,Yao, Bin,Xu, Feng,Wu, Ning-Feng,Fan, Yun-Liu.

[2]Molecular and biochemical characterization of a distinct tyrosinase involved in melanin production from Aeromonas media. Wan, Xia,Chai, Baozhong,Liao, Yi,Ye, Tao,Shen, Ping,Chen, Xiangdong,Wan, Xia,Su, Ying.

[3]Codon optimization of Bacillus licheniformis beta-1,3-1,4-glucanase gene and its expression in Pichia pastoris. Teng, Da,Fan, Ying,Yang, Ya-lin,Tian, Zi-gang,Luo, Jin,Wang, Jian-hua.

[4]A Na+/H+ antiporter gene of the moderately halophilic bacterium Halobacillus dabanensis D-8(T): cloning and molecular characterization. Yang, LF,Jiang, JQ,Zhao, BS,Zhang, B,Feng, DQ,Lu, WD,Wang, L,Yang, SS.

[5]A delta-endotoxin encoded in Pseudomonas fluorescens displays a high degree of insecticidal activity. Peng, R,Xiong, A,Li, X,Fuan, H,Yao, Q.

[6]Efficient recovery of the functional IP10-scFv fusion protein from inclusion bodies with an on-column refolding system. Guo, JQ,Li, QM,Zhou, JY,Zhang, GP,Yang, YY,Xing, GX,Zhao, D,You, SY,Zhang, CY.

[7]Expression of genes involved in progesterone receptor paracrine signaling and their effect on litter size in pigs. Chen, Xiao,Fu, Jinluan,Wang, Aiguo,Chen, Xiao,Fu, Jinluan,Wang, Aiguo,Chen, Xiao. 2016

[8]Comparison of cerato-platanin family protein BcSpl1 produced in Pichia pastoris and Escherichia coli. Liang, Yingbo,Qiu, Dewen,Yuan, Jingjing,Yang, Xiufen. 2017

[9]Heterologous expression of a gene encoding a thermostable beta-galactosidase from Alicyclobacillus acidocaldarius. Yuan, Tiezheng,Yang, Peilong,Wang, Yaru,Meng, Kun,Luo, Huiying,Yao, Bin,Zhang, Wei,Wu, Ningfeng,Fan, Yunliu.

[10]A novel highly acidic beta-mannanase from the acidophilic fungus Bispora sp MEY-1: gene cloning and overexpression in Pichia pastoris. Luo, Huiying,Wang, Yaru,Wang, Hui,Yang, Jun,Yang, Yuhui,Huang, Huoqing,Yang, Peilong,Bai, Yingguo,Shi, Pengjun,Yao, Bin,Fan, Yunliu.

[11]The degradation effects of a Pseudomonas hydrolase OPHC2 to organophosphorus insecticides. Wang, Jin-Fang,Gao, Ming-Hui,Pan, Can-Ping,Wu, Ning-Feng.

[12]Cloning, expression, purification, and characterization of an organophosphate-degrading enzyme in Escherichia coli. Wang, Jinbin,Chen, Dachao,Hu, Dongxiao,Su, Xuefeng,Tang, Xueming. 2013

[13]Expression, Identification and Characterize of CD25-Binding Epitope Modified Human IL-2 in Pichia pastoris. Wang, S.,Li, H. T.,Liu, Y. H.,Zhu, Y. Z.,Wang, W. H.,An, Y. X.,Miao, L. G..

[14]Soluble expression and enzymatic activity evaluation of protease from reticuloendotheliosis virus. Hu, Feng,Zhao, Yan,Qi, Xiaole,Cui, Hongyu,Gao, Yulong,Gao, Honglei,Liu, Changjun,Wang, Yongqiang,Zhang, Yanping,Li, Kai,Wang, Xiaomei,Wang, Yunfeng,Wang, Xiaomei,Wang, Yunfeng.

[15]Expression, purification and characterization of WSSV-VP37 in Pichia pastoris. Liu Qing-hui,Huang Jie,Han Wen-jun,Liang Yan,Lu Chun-ling,Wang Qing-yin. 2006

[16]Purification and Recombinant Expression of Major Peanut Allergen Ara h 1. Yan, Fei,Wei, Xiaonan,Li, Xin,Tong, Ping,Chen, Hongbing,Wu, Zhihua,Yang, Anshu,Chen, Hongbing,Yan, Fei,Tang, Ronghua.

[17]Inducible expression of calreticulin-N58 in Pichia pastoris by high density cell culture. Su, D. X.,Zhang, A. L.,Liu, Z. W.,Luo, J. X.,Zhang, T. Y.,Luo, J. X.,Zhang, T. Y.,Yi, G. H.,Rao, L. Y.,Zhou, Z. J..

[18]Expression of Prolactin Receptor mRNA after Melatonin Manipulated in Cashmere Goats Skin during Cashmere Growth. Zhang, Wei,Zhu, Xiaoping,Jia, Zhihai,Yue, Chunwang,Kong, Xianghao,Du, Lixin.

[19]Isolation and characterization of a cDNA encoding a papain-like cysteine protease from alfalfa. Yan, Longfeng,Han, Jianguo,Sun, Yan,Yan, Longfeng,Yang, Qingchuan,Kang, Junmei,Liu, Zhipeng,Wu, Mingsheng.

[20]Effects of age and strain on the expression of leptin receptor, neuropeptide Y and pro-opiomelanocortin in the hypothalamus of young chickens. Huang, J. X.,Lu, L.,Luo, X. G.,Liu, B.,Huang, J. X.,Lu, L.,Luo, X. G.,Liu, B.,Huang, J. X.,Xi, L..

作者其他论文 更多>>

微信小程序