An inactivated recombinant rabies virus chimerically expressed RBD induces humoral and cellular immunity against SARS-CoV-2 and RABV

文献类型： 外文期刊

第一作者： Zhang, Haili

作者： Zhang, Haili;Jin, Hongli;Song, Yumeng;Dai, Jiaxin;Jiao, Cuicui;Bai, Yujie;Sun, Jingxuan;Zhang, Mengyao;Huang, Jingbo;Huang, Pei;Li, Yuanyuan;Wang, Hualei;Yan, Feihu;Wang, Shen;Xia, Xianzhu;Jin, Hongli;Liu, Di;Lu, Jilong

作者机构： Jilin Univ, Coll Vet Med, Key Lab Zoonosis Res, Minist Educ, Changchun 130062, Peoples R China;Chinese Acad Agr Sci, Changchun Vet Res Inst, Changchun 130122, Peoples R China;Changchun Sino Biotechnol Co Ltd, Changchun 130012, Peoples R China

关键词： SARS-CoV-2; Vaccine; RBD; Chimeric expression; RABV

期刊名称：VIROLOGICA SINICA （ 2022影响因子：5.5； 五年影响因子：4.9 ）

ISSN： 1674-0769

年卷期： 2023 年 38 卷 2 期

页码：

收录情况： SCI

摘要： Many studies suggest that severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can infect various animals and transmit among animals, and even to humans, posing a threat to humans and animals. There is an urgent need to develop inexpensive and efficient animal vaccines to prevent and control coronavirus disease 2019 (COVID-19) in animals. Rabies virus (RABV) is another important zoonotic pathogen that infects almost all warmblooded animals and poses a great public health threat. The present study constructed two recombinant chimeric viruses expressing the S1 and RBD proteins of the SARS-CoV-2 Wuhan01 strain based on a reverse genetic system of the RABV SRV9 strain and evaluated their immunogenicity in mice, cats and dogs. The results showed that both inactivated recombinant viruses induced durable neutralizing antibodies against SARS-CoV-2 and RABV and a strong cellular immune response in mice. Notably, inactivated SRV-nCoV-RBD induced earlier antibody production than SRV-nCoV-S1, which was maintained at high levels for longer periods. Inactivated SRV-nCoV-RBD induced neutralizing antibodies against both SARS-CoV-2 and RABV in cats and dogs, with a relatively broadspectrum cross-neutralization capability against the SARS-CoV-2 pseudoviruses including Alpha, Beta, Gamma, Delta, and Omicron, showing potential to be used as a safe bivalent vaccine candidate against COVID-19 and rabies in animals.

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