Development, validation, and implementation of an ultratrace analysis method for the determination of moenomycin A, in aquatic animal products

文献类型： 外文期刊

第一作者： Tang, Yunyu

作者： Tang, Yunyu;Yang, Guangxin;Huang, Dongmei;Zhang, Xuan;Li, Siman;Shi, Yongfu;Kong, Cong;Ma, Yingqing;Zhang, Weiyi;Zhai, Wenlei;Fodjo, Essy Kouadio

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关键词： Moenomycin A; Aquatic animal products; HPLC-MS/MS; Drug residues; Dispersive solid-phase extraction; Validation

期刊名称：ANALYTICAL AND BIOANALYTICAL CHEMISTRY （ 影响因子：4.3； 五年影响因子：4.0 ）

ISSN： 1618-2642

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Moenomycin A, an antimicrobial growth promoter widely used as an additive in aquaculture feedstuffs, has been restricted for use in the European Union and China due to its potential risk of promoting resistant strains of pathogenic bacteria and causing residues in aquatic animal products. Although methods for analyzing moenomycin A in feedstuffs have been developed, no established method exists for aquatic matrices. In this study, we present, for the first time, a sensitive and validated high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method for the determination of moenomycin A in aquatic animal products. Samples were extracted using methanol and purified with the QuEChERS method employing C18 sorbent. The aliquot was dried under a nitrogen stream, reconstituted with methanol-water solvent, and analyzed by HPLC-MS/MS. The developed method exhibited good linearity (r(2) > 0.995) over a wide concentration range (1-100 mu g/L) and a low limit of detection (1 mu g/kg). Average recoveries ranged between 70 and 110% at spiked concentrations of 1, 50, and 100 mu g/kg, with associated intra- and inter-day relative standard deviations of 1.25 to 7.32% (n = 6) and 2.91 to 10.08% (n = 3), for different representative aquatic animal production, respectively. To the best of our knowledge, this is the first reported HPLC-MS/MS method for the quantification of moenomycin A in aquatic animal products. The new approach was effectively employed in the analysis of moenomycin A across various aquatic samples.

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