Impaired 2′,3′-cyclic phosphate tRNA repair causes thermo-sensitive genic male sterility in rice

文献类型： 外文期刊

第一作者： Yan, Bin

作者： Yan, Bin;Liu, Chunyan;Sun, Jing;Zhou, Can;Li, Ji;Liu, Wei;Li, Shengdong;Cao, Xiaofeng;Sun, Jing;Mao, Yang;Nie, Jiawei;Gao, Feng;Chen, Yuhang;Zhou, Can;Li, Ji;Li, Shengdong;Nie, Jiawei;Chen, Yuhang;Cao, Xiaofeng;Yan, Wei;Fu, Chenjian;Qin, Peng;Fu, Xingxue;Zhao, Xinghui;Yang, Yuanzhu;Song, Xianwei;Yang, Yuanzhu;Yang, Yuanzhu

作者机构：

期刊名称：CELL RESEARCH （ 影响因子：25.9； 五年影响因子：36.6 ）

ISSN： 1001-0602

年卷期： 2024 年 34 卷 11 期

页码：

收录情况： SCI

摘要： Hybrid rice, widely planted in Asia, is pathogen resistant and has superior yields, making it a major contributor to global food security. The two-line hybrid rice system, which utilizes mutants exhibiting photo-/thermo-sensitive genic male sterility (P/TGMS), is the leading hybrid rice breeding technology. Mutations in THERMO-SENSITIVE GENIC MALE STERILE 5 (TMS5) accounts for over 95% of current TGMS lines. We previously found that tms5 carries a mutation in ribonuclease ZS1. Despite its importance for breeding robust rice lines, the mechanism underlying tms5-mediated TGMS remains elusive. Here, we demonstrate that TMS5 is a tRNA 2 ',3 '-cyclic phosphatase. The tms5 mutation leads to accumulation of 2 ',3 '-cyclic phosphate (cP)-Delta CCA-tRNAs (tRNAs without 3 ' CCA ended with cP), which is exacerbated by high temperatures, and reduction in the abundance of mature tRNAs, particularly alanine tRNAs (tRNA-Alas). Overexpression of tRNA-Alas in the tms5 mutant restores male fertility to 70%. Remarkably, male fertility of tms5 mutant is completely restored at high temperatures by knocking out OsVms1 which encodes the enzyme for cP-Delta CCA-tRNA generation. Our study reveals the mechanism underlying tms5-mediated TGMS in rice and provides mechanistic insight into the further improvement of TGMS in hybrid crop development.

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