Adenovirus-vectored PDCoV vaccines induce potent humoral and cellular immune responses in mice

文献类型： 外文期刊

第一作者： Miao, Xin

作者： Miao, Xin;Zhang, Liping;Zhou, Peng;Yu, Ruiming;Zhang, Zhongwang;Wang, Cancan;Guo, Huichen;Wang, Yonglu;Pan, Li;Liu, Xinsheng;Miao, Xin;Zhang, Liping;Zhou, Peng;Yu, Ruiming;Zhang, Zhongwang;Wang, Cancan;Guo, Huichen;Wang, Yonglu;Pan, Li;Liu, Xinsheng;Miao, Xin;Zhang, Liping;Yu, Ruiming;Zhang, Zhongwang;Wang, Cancan;Pan, Li;Liu, Xinsheng;Pan, Li;Liu, Xinsheng

作者机构：

关键词： Ad5-vector vaccine; PDCoV; Spike glycoprotein; Oral; Immunogenicity

期刊名称：VACCINE （ 影响因子：5.5； 五年影响因子：4.3 ）

ISSN： 0264-410X

年卷期： 2023 年 41 卷 45 期

页码：

收录情况： SCI

摘要： Porcine deltacoronavirus (PDCoV) is a novel swine enteropathogenic coronavirus that causes severe watery diarrhea, vomiting, dehydration and high mortality in piglets, resulting in significant economic losses by the global pig industry. Recently, PDCoV has also shown the potential for cross-species transmission. However, there are currently few vaccine studies and no commercially available vaccines for PDCoV. Hence, here, two novel human adenovirus 5 (Ad5)-vectored vaccines expressing codon-optimized forms of the PDCoV spike (S) glycoprotein (Ad-PD-tPA-Sopt) and S1 glycoprotein (Ad-PD-oriSIP-S1opt) were constructed, and their effects were evaluated via intramuscular (IM) injection in BALB/c mice with different doses and times. Both vaccines elicited robust humoral and cellular immune responses; moreover, Ad-PD-tPA-Sopt-vaccinated mice after two IM injections with 108 infectious units (IFU)/mouse had significantly higher anti-PDCoV-specific neutralizing antibody titers. In contrast, the mice immunized with Ad-PD-tPA-Sopt via oral gavage (OG) did not generate robust systemic and mucosal immunity. Thus, IM Ad-PD-tPA-Sopt administration is a promising strategy against PDCoV and provides useful information for future animal vaccine development.

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