文献类型： 外文期刊

第一作者： Yang, Qing

作者： Yang, Qing;Lu, Yanhui;Yang, Qing;Lu, Yanhui

作者机构：

关键词： Hippodamia variegata; transcriptome; heat shock protein; RNA interference

期刊名称：INSECTS （ 影响因子：2.9； 五年影响因子：3.3 ）

ISSN：

年卷期： 2024 年 15 卷 9 期

页码：

收录情况： SCI

摘要： Hippodamia variegata is a key natural enemy of cotton-growing regions in northwestern China. In this study, transcriptome sequencing analysis was performed on H. variegata, after beetles were exposed to different temperatures (from 32 to 38 degree celsius) for 24 h, using high-throughput sequencing technology. We found the largest number of differentially expressed genes (DEGs) in the 35 degree celsius vs. 32 degree celsius group (1151); the fewest DEGs were found in the 38 degree celsius vs. 35 degree celsius group (901), indicating that H. variegata expressed the largest number of newly mobilized genes under medium-high temperature (35 degree celsius). Gene functional analysis showed that the DEGs were mainly involved in "Catalytic activity", "Oxidoreductase activity", "Metabolic pathways", and "Longevity regulating pathway-multiple species" gene groups. The results from qRT-PCR were consistent with the transcriptome data, confirming their reliability. Finally, the RNAi results showed that adult survival, longevity, and fecundity were lower in the group in which gene expression of the heat shock proteins (Hsp70-01 and Hsp68) was suppressed than in the control group (injection ds-GFP) at all the experimental temperatures (32, 35, and 38 degree celsius). Our results indicate the important role of the heat shock proteins (Hsp70-01 and Hsp68) in resistance to high-temperature stress in H. variegata and provide a molecular basis for analyzing the thermotolerance mechanism of H. variegata. Previous studies have shown that the survival and reproduction of Hippodamia variegata are increasingly harmed by progressive increases in temperature (from 32 degree celsius to 35 degree celsius and 38 degree celsius). In this study, transcriptome sequencing analysis was performed on H. variegata, after being exposed to different temperatures (from 32 to 38 degree celsius) for 24 h, using high-throughput sequencing technology. We found the largest number of differentially expressed genes (DEGs) in the 35 degree celsius vs. 32 degree celsius group (1151) followed by the 38 degree celsius vs. 32 degree celsius group (1054) and then the 38 degree celsius vs. 35 degree celsius group (901), indicating that H. variegata expressed the largest number of newly mobilized genes under medium-high temperature (35 degree celsius). Gene functional analysis showed that a large number of DEGs were involved in "Catalytic activity", "Oxidoreductase activity", "Metabolic pathways", and "Longevity regulating pathway-multiple species" gene groups. We randomly selected nine DEGs for validation using qRT-PCR. The results of qRT-PCR were consistent with the transcriptome data, confirming their reliability. Finally, the RNAi results showed that adult survival, longevity, and fecundity were lower in the group in which gene expression of the heat shock proteins (Hsp70-01 and Hsp68) was suppressed than in the control group (injection ds-GFP) at all the experimental temperatures (32, 35, and 38 degree celsius). Our results indicate the important role of the heat shock proteins (Hsp70-01 and Hsp68) in resistance to high-temperature stress in H. variegata and provide a molecular basis for analyzing its thermotolerance mechanism.

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