Genome-wide identification and expression profiling of duplicated flavonoid 3 '- hydroxylase gene family in Carthamus tinctorius L.

文献类型： 外文期刊

第一作者： Hoang, Nguyen Q., V

作者： Hoang, Nguyen Q., V;Jie, Kong;Ahmad, Naveed;Ma Xintong;Zhang Xinyue;Wu Yuhang;Nan, Wang;Na, Yao;Liu Xiuming;Liu Xiuming;Jin Libo;Ahmad, Naveed;Ahmad, Naveed;Liu Xiuming;Jin Libo

作者机构：

关键词： differential expression; flavonoid 3 '-hydroxylase (F3 ' H); prokaryotic expression; safflower; transient expression

期刊名称：NOTULAE BOTANICAE HORTI AGROBOTANICI CLUJ-NAPOCA （ 影响因子：1.444； 五年影响因子：1.363 ）

ISSN： 0255-965X

年卷期： 2021 年 49 卷 4 期

页码：

收录情况： SCI

摘要： Flavonoid 3'-hydroxylase (F3'H) enzyme is essential in determining the flavonoids B-ring hydroxylation pattern. It is mainly implicated in the biosynthetic pathway of cyaniding-based anthocyanins, flavonols, and flavan-3-ols. However, the evolution and regulatory mechanism of these important flavonoid hydroxylases have not been systematically investigated in safflower (Carthamus tinctorius L.). In this study, we identified 22 duplicated CtF3'-H- encoding genes from safflower through genome-wide prediction and conservation analysis. Phylogenetic analysis revealed the pattern of conservation and divergence of CtF3'Hs encoding proteins and their homologs from different plant species. The distribution of conserved protein motifs and cis-regulatory units suggested several structural components that could be crucial in deciphering the final function of CtF3'H proteins. Furthermore, the results of RNA-seq and qRT-PCR assay in different flowering tissues suggested differential expression level of CtF3'H genes during flower development. Based on the unique homology of CtF3'H5 with flavonoid 3' hydroxylases from other plant species, further validation of CtF3'H5 was carried out. The transient expression of CtF3'H5 in onion epidermal cells implied that the subcellular localization of the fusion construct containing CtF3'H5 and GFP was predominantly detected in the plasma membrane. Similarly, the prokaryotic expression and western blot hybridization of CtF3'H5 demonstrated the detection of a stable 50.3kD target protein. However, more efforts are needed to further extend the functional validation of CtF3'H5 in safflower. This study provides a fundamental gateway for future functional studies and understanding the genetic evolution of F3'Hs in plants.

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