Prostaglandin A(2) induces apoptosis in three cell lines derived from the fall armyworm, Spodoptera frugiperda

文献类型： 外文期刊

第一作者： Wang, Yong

作者： Wang, Yong;Goodman, Cynthia L.;Ringbauer, Joseph, Jr.;Stanley, David;Li, Yaofa

作者机构：

关键词： apoptosis; caspase; insect cell line; prostaglandins; Spodoptera frugiperda

期刊名称：ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：1.698； 五年影响因子：1.758 ）

ISSN： 0739-4462

年卷期： 2021 年 108 卷 3 期

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收录情况： SCI

摘要： Animals maintain homeostasis of cell numbers, constantly creating new cells and eliminating others. Programmed cell death, apoptosis, is a mechanism of cell elimination and it acts in many aspects of animal biology. Drawing on the biomedical background, several signals launch the apoptosis mechanisms, including prostaglandins (PGs). Based on this information, we posed the hypothesis that PGs similarly induce apoptosis in insect cell lines. We used three Spodoptera frugiperda cell lines, including two newly established, BCIRL-SfNS-0518B-YL derived from the central nervous system and BCIRL-Sf4FB-0614-SGS derived from fat body, and the commercially available Sf9 cells. Using a kinetic apoptosis kit, we found treating SfNS cells for 18 h with 15 or 20 mu M PGA(2) led to decreases in cell numbers, coupled with increased numbers of apoptotic and dead cells. Similar exposures to 10 mu M PGA(2) (24 h) led to substantial increases in apoptotic cells, confirmed by a terminal deoxynucleotidyl transferase dUTP nick end labeling assay on a flow cytometer. The influence of PGA(2) treatments increased with dosage, as we recorded about 20% apoptosis at 24 h post-PGA(2) treatments (10 mu M) and about 34% apoptosis at 24 h post-30 mu M treatments. PGA(2) treatments led to 10- to 30-fold increases in messenger RNAs (mRNAs) encoding apoptosis-specific caspases-1, -2, -3, and -5 at 12 h and 40- to 60-fold increases in mRNAs encoding caspases-1 and -2, 10-fold increases for caspases-3 and -5 at 24 h. These findings strongly support our hypothesis that PGs induce apoptosis in an insect cell line and confirm an additional PG action in insect biology.

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