Overlapping pathways involved in resistance against Sclerotinia stem rot in Brassica napus revealed through transcriptomic and metabolomic profiling

文献类型： 外文期刊

第一作者： Qasim, Muhammad Uzair

作者： Qasim, Muhammad Uzair;Zhao, Qing;Shahid, Muhammad;Huang, Huibin;Fan, Chuchuan;Zhou, Yongming;Cai, Guangqin;Li, Guoqing;Li, Guoqing

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关键词： Brassica napus L; Comparative transcriptomic; Metabolomic profiling; Sclerotinia stem rot; Resistance pathways

期刊名称：PLANT GROWTH REGULATION （ 影响因子：4.2； 五年影响因子：4.0 ）

ISSN： 0167-6903

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Brassica napus is one of the most important oilseed crops in the world, yet there are many challenges to its potential production. Sclerotinia sclerotiorum, which causes Sclerotinia stem rot, is a major fungal disease that reduces seed yield and oil quality. Research has been conducted to understand the genetic basis of resistance to Sclerotinia stem rot, with the majority of studies based on the identification of single or few resistant genes for disease control. The aim of this study was to identify genes involved in horizontal resistance to achieve a stable resistance response by investigating the key pathways adopted by the resistant lines in response to Sclerotinia stem rot. Therefore, we conducted comparative transcriptomic and metabolomic profiling to identify overlapping pathways in gene expression and biosynthesis. The resistance response was activated with the induction of the biosynthesis of amino acids and the biosynthesis of secondary metabolites in the resistant line. Early induction of phenylpropanoid biosynthesis at 24 hours post inoculation and arginine biosynthesis at three time points, i.e., 24, 48, and 96 hours post inoculation, induced specificity in resistant line. Glucosinolate biosynthesis, flavonoid biosynthesis, and the alanine, aspartate, and glutamate metabolism pathways were induced in the resistant line in our transcriptomics and metabolomics study. The genes for ethylene, salicylic acid, and jasmonic acid were also highly induced in the resistant line compared to the susceptible line based on the annotation of biotic stress genes using MapMan analysis. Our results may allow for the achievement of horizontal resistance and provide insight to breeders developing resistant varieties.

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