Transcriptomic Analysis of Sodium-Silicate-Induced Resistance against Rhizoctonia solani AG-3 in Potato

文献类型： 外文期刊

第一作者： Feng, Yayan

作者： Feng, Yayan;Zhang, Dongmei;Huo, Hongli;Li, Lele;Xiu, Zhijun;Yang, Chunfang;Zhang, Xiaoyu;Hao, Jianjun;Zhang, Dongmei;Huo, Hongli;Xiu, Zhijun;Yang, Chunfang

作者机构：

关键词： Solanum tuberosum; Rhizoctonia solani; sodium silicate; differentially expressed genes; RNA sequencing; transcriptome

期刊名称：AGRONOMY-BASEL （ 影响因子：3.3； 五年影响因子：3.7 ）

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年卷期： 2024 年 14 卷 6 期

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收录情况： SCI

摘要： Stem canker and black scurf of potatoes, caused by Rhizoctonia solani, are economically important diseases. Although the field application of sodium silicate has been shown to improve potato's resistance against R. solani, the underlying mechanism remains unclear. In this study, we examined this resistance using transcriptomic analysis. Potato stems inoculated with R. solani were treated with sodium silicate, while a control group received no sodium silicate treatment. The plants were grown under natural environmental conditions at the farm of Inner Mongolia Agricultural University. Potato stems were sampled 4, 8, and 12 days after treatment. Total RNA was extracted using the TRIzol reagent and transformed into cDNA. The cDNA was sequenced, the reads were aligned, and the expression levels of genes were quantified and compared between the treated and control groups. A total of 1491 genes were identified as differentially expressed genes (DEGs). Furthermore, these DEGs were found to be involved in hydrolase activity, plant-pathogen interactions, hormone signal transduction, and the phenylpropanoid biosynthesis pathway. To confirm the up- and down-regulation of DEGs, quantitative real-time polymerase chain reaction (qRT-PCR) was performed on randomly selected genes. The results showed that the application of sodium silicate induces a complex defense network in potato plants involving physical barriers, innate immunity, phytohormone signaling, and various phenylpropanoid compounds to combat R. solani infection. This study provides valuable insights into the molecular mechanisms underlying sodium-silicate-induced resistance and its potential for reducing stem canker and black scurf in potato crops.

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