Production of capsaicinoid nonivamide from plant oil and vanillylamine via whole-cell biotransformation

文献类型： 外文期刊

第一作者： Ge, Jianzhong

作者： Ge, Jianzhong;Zhang, Jie;Wang, Xiaolu;Bai, Yingguo;Wang, Yaru;Wang, Yuan;Tu, Tao;Qin, Xing;Su, Xiaoyun;Luo, Huiying;Yao, Bin;Huang, Huoqing;Huang, Huoqing

作者机构：

关键词： Capsaicinoid; Whole-cell biotransformation; Plant oil; Oleic acid; Nonanoic acid

期刊名称：BIORESOURCE TECHNOLOGY （ 影响因子：11.4； 五年影响因子：10.6 ）

ISSN： 0960-8524

年卷期： 2023 年 390 卷

页码：

收录情况： SCI

摘要： Capsaicinoids are mostly derived from chili peppers and have widespread applications in food, feed, and pharmacology. Compared with plant extraction, the use of microbial cell factories for capsaicinoids production is considered as a more efficient approach. Here, the biotransformation of renewable plant oil and vanillylamine into capsaicinoid nonivamide was investigated. Nonivamide biosynthesis using nonanoic acid and vanillylamine as substrates was achieved in Escherichia coli by heterologous expression of genes encoding amide-forming N- acyltransferase and CoA-ligase. Through increasing nonanoic acid tolerance of chassis cell, screening key enzymes involved in nonivamide biosynthesis and optimizing biotransformation conditions, the nonivamide titer reached 0.5 g/L. By further integrating a route for conversion of oleic acid to nonanoic acid, nonivamide biosynthesis was finally achieved using olive oil and vanillylamine as substrates, yielding a titer of approximately 10.7 mg/L. Results from this study provide valuable information for constructing highly efficient cell factories for the production of capsaicinoid compounds.

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