Engineered Exosomes Delivering bta-miR-223 Alleviate Staphylococcus aureus-Induced Mastitis in Mice via the TLR4/NF-κB Signaling Pathway

文献类型： 外文期刊

第一作者： Wang, Yuxuan

作者： Wang, Yuxuan;Ouyang, Kun;Nie, Ying;Yang, Jingshuo;Liao, Yunxin;Wang, Hailong;Luo, Junyi;Sun, Jiajie;Xi, Qianyun;Chen, Ting;Zhang, Yongliang;Chen, Xingping;Shen, Binglei

作者机构：

关键词： bovine mastitis; engineered exosomes; bta-miR-223; nanotherapeutics; Staphylococcus aureus

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.2； 五年影响因子：6.4 ）

ISSN： 0021-8561

年卷期： 2025 年 73 卷 28 期

页码：

收录情况： SCI

摘要： Bovine mastitis is an inflammatory disease of the mammary gland, frequently associated with infection by Staphylococcus aureus. Effective delivery of the antiinflammatory microRNA bta-miR-223 remains a challenge. In this study, we constructed engineered exosomes loaded with bta-miR-223 and demonstrated their antiinflammatory effects both in vitro (Mac-T cells) and in vivo (mice). Overexpression of bta-miR-223 reduced the expression of IL-6 and IL-1 beta in a lipoteichoic acid-induced Mac-T cell inflammation model. Following tail vein injection in lactating mice, the engineered exosomes accumulated in the mammary gland, alleviated S. aureus-induced inflammation, and increased the expression of barrier-related proteins ZO-1, claudin-1, and occludin. Mechanistically, bta-miR-223 inhibited RHOB expression and modulated the TLR4/NF-kappa B pathway. These results demonstrate that exosome-mediated delivery of bta-miR-223 effectively alleviates mammary inflammation, providing a novel strategy for nucleic acid nanotherapy.

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