Separation and identification of neutral oligosaccharides with prebiotic activities from apple pectin

文献类型： 外文期刊

第一作者： Liu, Huipeng

作者： Liu, Huipeng;Wei, Xiaoyu;Zu, Shuyu;He, Ning;Zhang, Jinchuang;Shi, Aimin;Wang, Qiang;Lin, Xihuang

作者机构：

关键词： Neutral oligosaccharides; UPLC-ESI-MS/MS; Lactobacillus. strains; Prebiotic potential

期刊名称：FOOD HYDROCOLLOIDS （ 影响因子：9.147； 五年影响因子：9.169 ）

ISSN： 0268-005X

年卷期： 2021 年 121 卷

页码：

收录情况： SCI

摘要： Plant-derived neutral oligosaccharides and pectic-oligosaccharides (POS) have been suggested as prebiotics based on their degree of polymerization (DP) and structural complexity. In the present study, neutral oligosaccharides without galacturonic acid were produced from commercial apple pectin using an optimized concentration of pectinase and degradation time. The crude oligosaccharide solution was refined by membrane separation using a DEAE-Sepharose CL-6B column, a graphite carbon column and an Xbridge C18 column, which resulted in the neutral oligosaccharides with DP 3-13. The monosaccharide composition of oligosaccharides primarily included glucose, arabinose, galactose and xylose without galacturonic acid. The structure of the oligosaccharides was analyzed using mass spectrometry with an electrospray ionization source by UPLC on an HILIC column and H-1 NMR. The MS/MS spectra in positive ion mode and H-1 NMR showed a main oligosac- charide structure corresponding to the connection of arabinogalactan with Gal beta 1-4Gal beta 1 -> 4Gal beta 1 -> 4Gal beta 1 ->alpha 3Araf. The growth of the selected Lactobacillus. strains was improved by adding refined neutral oligosaccharides with DP = 3-13 compared with commercial inulin, and growth of Escherichia coli had not basically grown. Therefore, neutral oligosaccharides (DP = 3-13) can be considered effective prebiotics for potential application in functional food.

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