Molecular mechanisms regulating glucose metabolism in quinoa (Chenopodium quinoa Willd.) seeds under drought stress

文献类型： 外文期刊

第一作者： Wang, Chunmei

作者： Wang, Chunmei;Wang, Junling;Liu, Xiaoqing;Wang, Xiaoxia;Wei, Boxiang;Mu, Guojun;Lu, Chuan;Qin, Yan;Lv, Wei;Wang, Chunmei;Wang, Junling;Liu, Xiaoqing;Wang, Xiaoxia;Wei, Boxiang;Mu, Guojun;Wei, Zhimin;Zhang, Huilong

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关键词： DEGs; DAMs; Transcriptome; Metabolome; Yeast high-throughput

期刊名称：BMC PLANT BIOLOGY （ 影响因子：4.3； 五年影响因子：5.2 ）

ISSN： 1471-2229

年卷期： 2024 年 24 卷 1 期

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收录情况： SCI

摘要： Background Abiotic stress seriously affects the growth and yield of crops. It is necessary to search and utilize novel abiotic stress resistant genes for 2.0 breeding programme in quinoa. In this study, the impact of drought stress on glucose metabolism were investigated through transcriptomic and metabolomic analyses in quinoa seeds. Candidate drought tolerance genes on glucose metabolism pathway were verified by qRT-PCR combined with yeast expression system. Results From 70 quinoa germplasms, drought tolerant material M059 and drought sensitive material M024 were selected by comprehensive evaluation of drought resistance. 7042 differentially expressed genes (DEGs) were indentified through transcriptomic analyses. Gene Ontology (GO) analysis revealed that these DEGs were closely related to carbohydrate metabolic process, phosphorus-containing groups, and intracellular membrane-bounded organelles. The Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis detected that DEGs were related to pathways involving carbohydrate metabolisms, glycolysis and gluconeogenesis. Twelve key differentially accumulated metabolites (DAMs), (D-galactose, UDP-glucose, succinate, inositol, D-galactose, D-fructose-6-phosphate, D-glucose-6-phosphate, D-glucose-1-phosphate, dihydroxyacetone phosphate, ribulose-5-phosphate, citric acid and L-malate), and ten key candidate DEGs (CqAGAL2, CqINV, CqFrK7, CqCELB, Cqbg1x, CqFBP, CqALDO, CqPGM, CqIDH3, and CqSDH) involved in drought response were identified. CqSDH, CqAGAL2, and Cq beta-GAL13 were candidate genes that have been validated in both transcriptomics and yeast expression screen system. Conclusion These findings provide a foundation for elucidating the molecular regulatory mechanisms governing glucose metabolism in quinoa seeds under drought stress, providing insights for future research exploring responses to drought stress in quinoa.

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