Metagenomics reveals the increased antibiotics resistome through prokaryote rather than virome after overuse of rare earth element compounds

文献类型： 外文期刊

第一作者： Song, Alin

作者： Song, Alin;Si, Zhiyuan;Sun, Miaomiao;Zhang, Jiayin;Wang, Sai;Wang, Enzhao;Bi, Jingjing;Fan, Fenliang;Peng, Jingjing;Xu, Duanyang;Chong, Fayao;Fan, Fenliang

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关键词： Rare earth; Virus; Metal resistance gene; Antibiotics resistance gene; Metagenomics

期刊名称：SCIENCE OF THE TOTAL ENVIRONMENT （ 影响因子：9.8； 五年影响因子：9.6 ）

ISSN： 0048-9697

年卷期： 2023 年 863 卷

页码：

收录情况： SCI

摘要： Rare earth elements (REE) are extensively exploited in the agricultural ecosystems due to their various beneficial roles on plant growth. However, the ecotoxicological effects and environmental risk of REE are poorly assessed. Here, we inves-tigated the effects of lanthanum and cerium nitrate on soil prokaryote and viral metal resistance genes (MRGs) and anti-biotics resistance genes (ARGs) using a metagenomic-based approach. We found that relative abundances of prokaryote phyla Bacteroidetes and Chloroflexi decreased with increasing of both REE compounds. In addition, low level REE nitrate (0.05 and 0.1 mmol kg-1 soil) inhibited the viral family Phycodanaviridae, Rudiviridae, Schitoviridae, whereas high level (0.16 and 0.32 mmol kg-1 soil) REE nitrate suppressed the viral family Herelleviridae, Iridoviridae, Podoviridae. ARGs were not significantly affected by low level of REE nitrate. However, high level of both REEs nitrate increased the abundances of dominant prokaryote genes resisting to most of the drug classes, such as aminoglycoside, elfamycin, fluoroquinolone, macrolide, rifamycin. Abundance of MRGs in prokaryote did not change consistently with REE nitrate compound type and input rate. MRGs were only partially detected in the virome in some of the treatments, while ARGs was not detected in virome. Together, we demonstrated that overuse of REE nitrate in agriculture would increase the risk of dissemination of ARGs through prokaryotes but not virus, although viral community was substantially shifted.

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