Antimicrobial potential of carvacrol against Edwardsiella piscicida in vitro

文献类型： 外文期刊

第一作者： Qin, Ting

作者： Qin, Ting;Dowah, Richline Gedeh Wentee;Xi, Bingwen;Xie, Jun;Qin, Ting;Chen, Kai;Xi, Bingwen;Pan, Liangkun;Xie, Jun;Dowah, Richline Gedeh Wentee

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关键词： Carvacrol; Edwardsiella piscicida; Biofilm formation; Hemolytic activity; Anti-virulence

期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.5； 五年影响因子：3.6 ）

ISSN： 0882-4010

年卷期： 2024 年 196 卷

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收录情况： SCI

摘要： With the alarming rise of antibiotic-resistant bacteria, novel antibacterial substances are urgently needed for controlling and treating multidrug-resistant bacterial infections. Edwardsiella piscicida is an important zoonotic enteric pathogen, that can cause systemic hemorrhagic septicemia in fish. Carvacrol, a major terpene of oregano essential oil, has a wide range of antibacterial activities. This study aimed to analyze the effect of carvacrol on the growth and virulence of E. piscicida in vitro. . The minimum inhibitory concentration (MIC) of carvacrol against E. piscicida was 125 mu g/mL. The sub-inhibitory concentrations of carvacrol significantly decreased the biofilm formation of E. piscicida in a dose dependent manner, whereas increased the hemolytic activity with a negative correlation. The quantitative real-time PCR results showed that carvacrol at sub-MICs downregulated the expression of related virulence genes, including flagellum ( fimA , fliC, flgN), ), hemolysins ( ethA, ethB), ), quorum sensing systems ( luxR, qseB), ), T3SS ( esrB, esrC) ) and T6SS ( evpB, evpC). ). Moreover, carvacrol (<= 1/8 <= 1/8 MIC) reduced the cytotoxicity, adherence and internalization activities of E. piscicida to the EPC cells. In vivo trial, the diet mixed with carvacrol increased the survival of zebrafish infected with E. piscicida. . Overall, these findings suggested that carvacrol might be a promising therapeutic agent against E. piscicida infection in aquaculture.

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