Establishment and characterization of a new intrahepatic cholangiocarcinoma cell line, ICC-X3

文献类型： 外文期刊

第一作者： Xu, Hao

作者： Xu, Hao;Luo, Wei;Zhao, Zhenjie;Chai, Changpeng;Hu, Jinjing;Miao, Xin;Tang, Huan;Zhou, Wence;Zhang, Hui;Zhou, Wence

作者机构：

关键词： Intrahepatic cholangiocarcinoma; Cell line; Establishment; Xenograft; Drug resistance

期刊名称：HUMAN CELL （ 影响因子：4.3； 五年影响因子：4.2 ）

ISSN： 0914-7470

年卷期： 2023 年 36 卷 2 期

页码：

收录情况： SCI

摘要： Intrahepatic cholangiocarcinoma (ICC) is an aggressive cancer of the biliary tract that is prone to recurrence and metastasis and is characterized by poor sensitivity to chemotherapy and overall prognosis. To address this challenge, the establishment of suitable preclinical models is critical. In this study, we successfully established a new ICC cell line, named ICC-X3, from the satellite lesions of one ICC patient. The cell line was characterized with respect to phenotypic, molecular, biomarker, functional and histological properties. STR confirmed that ICC-X3 was highly consistent with primary tumor tissue. ICC-X3 cells positively expressed CK7, CK19, E-cadherin, vimentin, and Ki67. ICC-X3 was all resistant to gemcitabine, paclitaxel, 5-FU, and oxaliplatin. The cell line was able to rapidly form xenograft tumors which were highly similar to the primary tumor. The missense mutation of TP53 exon was detected in ICC-X3 cells. ICC-X3 can be used as a good experimental model to study the progression, metastasis, and drug resistance of ICC.

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