Rapid Visual LAMP Method for Detection of Genetically Modified Organisms

文献类型： 外文期刊

第一作者： Xing, Yujun

作者： Xing, Yujun;Xu, Jianhong;Liang, Jie;Dong, Fei;Wu, Jirong;Shi, Jianrong;Wang, Jinke

作者机构：

期刊名称：ACS OMEGA （ 影响因子：4.1； 五年影响因子：4.0 ）

ISSN： 2470-1343

年卷期： 2023 年 8 卷 32 期

页码：

收录情况： SCI

摘要： We developed a novel loop-mediated isothermal amplification(LAMP)method using DNA captured on polyacrylamide microparticles (PAMMPs)as templates (PAMMPs@DNA-LAMP) for rapid qualitative detection ofgenetically modified organisms (GMOs). Here, DNA was extracted bya fast and cost-effective method using PAMMPs. Four LAMP primers weredesigned for the PAMMPs@DNA-LAMP method to detect the cauliflowermosaic virus 35S (CaMV35S) promotor in GMOs. We thus developed thismethod for rapid extraction of DNA (5-10 min) and fast amplificationof DNA within & SIM;30 min at a constant temperature of 63 & DEG;C.Moreover, the DNA captured by PAMMPs (PAMMPs@DNA) could be effectivelydetected by both conventional and quantitative PCR (qPCR) and LAMP.The PAMMPs@DNA-LAMP method was validated with high specificity, sensitivity,and performance for practical sample analysis. This assay detected0.01% target sequences, which had a high specificity like qPCR andbetter than the conventional PCR (cPCR). Furthermore, PAMMPs@DNA-LAMPwas successfully used to extract and detect DNA from food samplesof the major crops (soybean, maize, rice, etc.). In summary, a novelPAMMPs@DNA-LAMP assay has been developed, which has higher sensitivityand spends less time than the cPCR detection using the conventionalDNA extracted process. This method offers a novel approach for rapiddetection of GMOs in the field.

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