Reference Gene Selection for Analyzing the Transcription Patterns of Two Fatty Acyl-CoA Reductase Genes From Paracoccus marginatus (Hemiptera: Pseudococcidae)

文献类型： 外文期刊

第一作者： Liang, Xiao

作者： Liang, Xiao;Chen, Qing;Wu, Chunling;Liu, Ying;Liang, Xiao;Chen, Qing;Wu, Chunling;Liu, Ying;Liang, Xiao;Chen, Qing;Wu, Chunling;Liu, Ying;Han, Zhiling;Wu, Mufeng

作者机构：

关键词： Paracoccus marginatus; fatty acyl-CoA reductase gene; reference gene; cassava cultivar; transcription analysis

期刊名称：JOURNAL OF INSECT SCIENCE （ 影响因子：1.857； 五年影响因子：1.904 ）

ISSN：

年卷期： 2021 年 21 卷 5 期

页码：

收录情况： SCI

摘要： Paracoccus marginatus (Hemiptera: Pseudococcidae), known as the papaya mealybug, could cause considerable yield loss of several plants. To date, there is no molecular-based study of P. marginatus. Fatty acyl-CoA reductases (FARs) are key enzymes involved in wax synthesis. In the present study, we cloned and characterized coding sequences (CDS) of two FAR genes from P. marginatus. The results showed that PmFAR1 and PmFAR2 CDS were 1,590 and 1,497 bp in length, respectively, and sequence analysis indicated that these two genes both had the conservative motifs belonging to FAR_C superfamily. Furthermore, seven candidate reference genes were analyzed for their expression stability by using common algorithms including comparative Delta Cq method, geNorm, NormFinder, BestKeeper, and RefFinder. Eventually, beta-actin and GAPDHwere the best reference genes in evaluating the expression of those two FAR genes. We found that PmFAR1 and PmFAR2 showed distinct expression patterns in different life stages. Moreover, the transcription of PmFAR1 and PmFAR2 in P. marginatus fed on resistant cassava cultivars was significantly lower compared with those fed on susceptible ones, indicating the potential function of FAR genes in cassava resistance to P. marginatus. The present study might help in better understanding the molecular mechanism of cassava resistance to mealybug.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序