FCV activates NLRP3 inflammasome through ion-dependent pathway to promote IL-1β secretion

文献类型： 外文期刊

第一作者： Chen, Hongtao

作者： Chen, Hongtao;Liu, Shanling;Wei, Yiming;Gao, Kun;Zhang, Shuang;Wang, Kai;Hu, Guixue;Zhao, Shihui;Guo, Yanbing;Shao, Hongze;Zhao, Han;Yi, Shushuai;Bao, Di

作者机构：

关键词： FCV1; NLRP3 inflammasome 2; Cell pyroptosis 3; IL-1 beta 4

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：2.7； 五年影响因子：2.9 ）

ISSN： 0378-1135

年卷期： 2025 年 307 卷

页码：

收录情况： SCI

摘要： Feline calicivirus (FCV) poses a significant threat to the health of susceptible animals in the global epidemic. The inflammasome plays a crucial role in the inflammatory process induced by pathogens, functioning in resisting their invasion, responding to cellular damage, and maintaining internal environmental homeostasis.FCV infection in cats causes inflammation,and the regulatory mecha-nisms of the NLRP3 inflammasome on inflammatory factors in infected cells urgently require further investigation.This study focuses on this inflammasome as the entry point, focusing on its activation signal that activates IL-1 beta secretion.Through cellular experiments, it analyzes the molecular mechanisms and logical relationships between this inflammasome, intracellular environment, pyroptosis, the regulation of IL-1 beta secretion by viral-encoded proteins.The results showed that FCVinfected cells induced the activation and assembly of the NLRP3 inflammasome, thereby activating this signaling. FCV-induced IL-1 beta secretion was dependent on the activation of this inflammasome. In the early stages of infection, the activation of this inflammasome and IL-1 beta secretion were dependent on cellular Ca2 + influx and K+ efflux. IL-1 beta secretion also relied on FCV-induced pyroptosis;The activation of this inflammasome by the FCVencoded protein p32/LC and the subsequent induction of IL-1 beta secretion are contingent upon the function of its in mediating cellular Ca2+ influx and K+ efflux.In summary, this study demonstrates that following FCV infection of cells, its p32 and LC proteins activate the NLRP3 inflammasome activation signal in a manner dependent on Ca2+ influx and K+ efflux,Induce IL-1 beta secretion. In addition, FCV-induced pyroptosis also triggers the secretion of IL-1 beta. The findings of this study elucidate the leading to inflammatory mechanisms of FCV from a novel perspective, providing new drug targets for the design and screening of anti-inflammatory drugs.

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