Study on the optimization of the enzymatic hydrolysis of antimicrobial protein from Moringa oleifera Leaves by response surface method

文献类型： 外文期刊

第一作者： Huang, Zhilian

作者： Huang, Zhilian;Kang, Dandan;Huang, Zhilian;Kang, Dandan;Li, Jihua;Peng, Shaodan;Li, Jihua;Peng, Shaodan;Guo, Changqing

作者机构：

期刊名称：2019 5TH INTERNATIONAL CONFERENCE ON ADVANCES IN ENERGY RESOURCES AND ENVIRONMENT ENGINEERING (ICAESEE 2019)

ISSN： 1755-1307

年卷期： 2020 年 446 卷

页码：

收录情况： SCI

摘要： Moringa oleifera leaves contains antimicrobial protein, which is absent of optimized extraction and systematic analysis. The aim of this work is to provide a systematic research of its protein isolate hydrolysate on the optimized extraction via the response surface method and the functional properties. The isolated protein was obtained by alkali extraction and acid precipitation, which purity was 81.12%+/- 0.03%. Through the response surface method,the result of the optimization obtained the enzymatic hydrolysis of antimicrobial protein was as follows, the substrate concentration of 5.09%, the Bromelain enzyme dosage of 3883.42 U/g, pH 7.0 and temperature of 55 degrees C for 3.64 h. And the protein hydrolysate was indicated that the purity was 88.59%+/- 0.13%. Moreover, the ash,the crude fat and moisture were declined significantly compared with the protein isolate. This subject could provide basic theoretical basis for the application of M. oleifera leaves protein.

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