Flexible control of influx for flavonoids pathway based on diversity of MaCHI-fold proteins in mulberry

文献类型： 外文期刊

第一作者： Fu, Rumeng

作者： Fu, Rumeng;Yang, Guang;Zheng, Longyan;Huang, Shuai;Wang, Xinlei;Li, Ling;Li, Mengqi;Zhang, Dongyang;Cao, Xu;Chao, Nan;Liu, Li;Li, Mengqi;Cao, Xu;Chao, Nan;Liu, Li

作者机构：

关键词： Alternative transcript; Chalcone isomerase; Flavonoids; Metabolon; Mulberry

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）

ISSN： 0141-8130

年卷期： 2025 年 308 卷

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收录情况： SCI

摘要： Mulberry (Morus spp., Moraceae) is considered as a rapidly evolving plant species with enriched unique gene family members involved in flavonoid biosynthesis. Recently, transcriptional regulatory mechanism controlling flavonoid biosynthesis in mulberry has been extensively reported, yet studies on the regulation of flavonoid biosynthesis beyond the transcriptional level are limited. In present study, our in vivo and in vitro assays have shown that four MaCHI-fold proteins with different roles in flavonoid biosynthesis coordinated to flexibly regulate the influx for flavonoid biosynthesis. MaCHI2N was the dominant CHI to ensure the normal influx. The mulberry-specific type IB MaCHI1 worked as substitute and could also enhance the influx. The MaCHI2 alternative transcript product, MaCHI2D with low activity for naringin chalcone provided a "braker" for flavonoid biosynthesis by competing with MaCHI2N in transcription process. MaCHIL enhanced the influx for flavonoid biosynthesis by interacting with MaCHS1 and increasing the production of naringenin. Therefore, we proposed a transmission box model that presented the complicated regulation of influx for flavonoid biosynthesis based on the diversity of MaCHI-fold proteins and the homeostasis of naringenin chalcone and naringenin in mulberry. Our results reveal the unique regulatory mechanism of influx for flavonoids pathway beyond transcriptional level

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