Acute, subacute oral toxicity and Ames test of Py-mulin: an antibacterial drug candidate

文献类型： 外文期刊

第一作者： Fan, Yuan

作者： Fan, Yuan;Zhou, Yuhang;Liu, Yu;Hao, Baocheng;Shang, Ruofeng;Fu, Yunxing

作者机构：

关键词： Py-mulin; Acute toxicity; Subacute toxicity; Ames test

期刊名称：BMC PHARMACOLOGY & TOXICOLOGY （ 影响因子：2.605； 五年影响因子：2.943 ）

ISSN：

年卷期： 2022 年 23 卷 1 期

页码：

收录情况： SCI

摘要： Background Py-mulin is a new pleuromutilin derivative with potent antibacterial activities in vitro and in vivo, suggesting this compound may lead to a promising antibacterial drug after further development. The present study is aimed to evaluate the acute and subacute oral toxicity, and the genotoxicity with the standard Ames test according to standard protocols. Methods Acute oral toxicity of Py-mulin was determined using Kunming mice. The 28-day repeated dose oral toxicity study in SD rats was performed according to OECD guideline No. 407. The bacterial reverse mutation (Ames test) was carried out using four Salmonella typhimurium (S. typhimurium) strains TA97, TA98, TA100 and TA1535 with and without S9 metabolic activation. Results The LD50 values in acute oral toxicity were 2973 mg/kg (female mice) and 3891 mg/kg (male mice) calculated by the Bliss method. In subacute toxicity study, 50 mg/kg Py-mulin did not induce any abnormality in body weight, food consumption, clinical sign, hematology, clinical chemistry, organ weight, and histopathology in all of the treatment groups. However, high doses of Py-mulin (100 and 300 mg/kg) displayed slightly hepatotoxicity to female rats. Furthermore, Py-mulin did not significantly increase the number of revertant colonies of four standard S. typhimurium strains with the doses of 0.16-1000 mu g/plate in the Ames study. Conclusions Based on our findings, our study provides some information for the safety profile of Py-mulin.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序