Generation of rat forebrain tissues in mice

文献类型： 外文期刊

第一作者： Huang, Jia

作者： Huang, Jia;He, Bingbing;Li, Leijie;Li, Shuaishuai;Wu, Jun;Huang, Jia;He, Bingbing;Wei, Yinghui;Gao, Yanxia;Fang, Yuan;Ying, Wenqin;Wang, Zikang;Li, Chao;Zhou, Yingsi;Li, Shuaishuai;Choi, Seungwon;Zhou, Haibo;Yang, Hui;Yang, Xiali;Long, Xin;Guo, Fan;Long, Xin;Guo, Fan;Tang, Min;Choi, Seungwon;Shi, Linyu;Wu, Jun;Wu, Jun

作者机构：

期刊名称：CELL （ 影响因子：64.5； 五年影响因子：57.5 ）

ISSN： 0092-8674

年卷期： 2024 年 187 卷 9 期

页码：

收录情况： SCI

摘要： Interspecies blastocyst complementation (IBC) provides a unique platform to study development and holds the potential to overcome worldwide organ shortages. Despite recent successes, brain tissue has not been achieved through IBC. Here, we developed an optimized IBC strategy based on C-CRISPR, which facilitated rapid screening of candidate genes and identified that Hesx1 deficiency supported the generation of rat forebrain tissue in mice via IBC. Xenogeneic rat forebrain tissues in adult mice were structurally and functionally intact. Cross -species comparative analyses revealed that rat forebrain tissues developed at the same pace as the mouse host but maintained rat -like transcriptome profiles. The chimeric rate of rat cells gradually decreased as development progressed, suggesting xenogeneic barriers during mid -to -late pre -natal development. Interspecies forebrain complementation opens the door for studying evolutionarily conserved and divergent mechanisms underlying brain development and cognitive function. The C-CRISPR-based IBC strategy holds great potential to broaden the study and application of interspecies organogenesis.

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