Co-overexpression of AVP1, PP2A-C5, and AtCLCc in Arabidopsis thaliana greatly increases tolerance to salt and drought stresses

文献类型： 外文期刊

第一作者： Balasubramaniam, Thuvaraki

作者： Balasubramaniam, Thuvaraki;Wijewardene, Inosha;Hu, Rongbin;Zhang, Hong;Shen, Guoxin;Zhang, Jinlin

作者机构：

关键词： Chloride channel protein; Drought stress; Protein phosphatase 2A; Pyrophosphatase; Salt stress

期刊名称：ENVIRONMENTAL AND EXPERIMENTAL BOTANY （ 影响因子：6.028； 五年影响因子：6.246 ）

ISSN： 0098-8472

年卷期： 2022 年 200 卷

页码：

收录情况： SCI

摘要： Abiotic stresses such as salinity and drought impose a severe constraint on global food production, posing a serious challenge in agriculture. Stacking beneficial genes in transgenic crops will likely improve crop yield under abiotic stress conditions. Previous studies showed that individually overexpressing the Arabidopsis vacuolar H+-pyrophosphatase gene AVP1, the protein phosphatase 2 A catalytic subunit gene PP2A-C5, and the chloride channel protein gene AtCLCc contributed to enhanced salt tolerance and overexpression of AVP1 alone could also improve drought tolerance. We hypothesized that co-overexpressing AVP1, PP2A-C5, and AtCLCc, would combine the benefits of these three genes, leading to a further increase in salt tolerance in transgenic plants due to the potential synergism of these genes. Indeed, co-overexpression of these three genes in Arabi-dopsis significantly improved salt and drought tolerance under single as well as under combined salt and drought stresses. The AVP1/PP2A-C5/AtCLCc co-overexpressing plants displayed robust growth and produced greater amount of biomass as well as viable seeds than wild-type and single gene overexpression plants under saline and drought conditions. This study demonstrates that successful co-overexpression of several well-chosen genes is an effective strategy to achieve greater abiotic stress tolerance and could potentially lead to higher crop yield in regions of the world with saline soil and low precipitation.

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